Structural differences in the cell binding region of human fibronectin molecules isolated from cultured normal and tumor-derived human cells

Laura Borsi, Giorgio Allemanni, Patrizia Castellani, Claudio Rosellini, Angela Di Vinci, Luciano Zardi

Research output: Contribution to journalArticle

Abstract

Fibronectins isolated from human plasma (pFN) and from the conditioned media of normal (N-cFN) and tumor (T-cFN) human cells were compared by cathepsin D digestion followed by immunostaining of released fragments with the monoclonal antibody 3E3, specific for the cell binding site. Two different staining patterns were obtained, one specific for pFN and N-cFN, the second common to fibronectins from the 3 different kinds of tumors studied. This indicates structural differences between N-cFN and T-cFN in the cell binding region of the fibronectin molecule.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalFEBS Letters
Volume192
Issue number1
DOIs
Publication statusPublished - Nov 11 1985

Keywords

  • Cell binding site
  • Fibronectin
  • Immunoblotting
  • Tumor cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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