Structural stability of green fluorescent proteins entrapped in polyelectrolyte nanocapsules

Silke Krol, Fabio Cannone, Michele Caccia, Laura Sironi, Paolo Bianchini, Barbara Campanini, Maddalena Collini, Giuseppe Chirico, Alberto Diaspro

Research output: Contribution to journalArticlepeer-review

Abstract

Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein-polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule protective effect on GFPmut2 is likely due to protein interactions with the negatively charged polymers, that induce an increase in the local rigidity of the protein nano-environment. This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers.

Original languageEnglish
Pages (from-to)310-319
Number of pages10
JournalJournal of Biophotonics
Volume1
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • Fluorescence spectroscopy
  • FRET
  • GFP
  • Layer-by-layer
  • Polyelectrolyte
  • Protein denaturation

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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