Structure and function of the intracellular region of the plexin-B1 transmembrane receptor

Yufeng Tong, Prasanta K. Hota, Junia Y. Penachioni, Mehdi B. Hamaneh, SoonJeung J. Kim, Rebecca S. Alviani, Limin Shen, Hao He, Wolfram Tempel, Luca Tamagnone, Hee Won Park, Matthias Buck

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the plexin family are unique transmembrane receptors in that they interact directly with Rho family small GTPases; moreover, they contain a GTPase-activating protein (GAP) domain for R-Ras, which is crucial for plexin-mediated regulation of cell motility. However, the functional role and structural basis of the interactions between the different intracellular domains of plexins remained unclear. Here we present the 2.4Å crystal structure of the complete intracellular region of human plexin-B1. The structure is monomeric and reveals that the GAP domain is folded into one structure from two segments, separated by the Rho GTPase binding domain (RBD). The RBD is not dimerized, as observed previously. Instead, binding of a conserved loop region appears to compete with dimerization and anchors the RBD to the GAP domain. Cell-based assays on mutant proteins confirm the functional importance of this coupling loop. Molecular modeling based on structural homology to p120GAP·H-Ras suggests that Ras GTPases can bind to the plexin GAP region. Experimentally, we show that the monomeric intracellular plexin-B1 binds R-Ras but not H-Ras. These findings suggest that the monomeric form of the intracellular region is primed for GAP activity and extend a model for plexin activation.

Original languageEnglish
Pages (from-to)35962-35972
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number51
DOIs
Publication statusPublished - Dec 18 2009

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Structure and function of the intracellular region of the plexin-B1 transmembrane receptor'. Together they form a unique fingerprint.

Cite this