Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes

Nezha Laraki, Moreno Galleni, Iris Thamm, Maria Letizia Riccio, Gianfranco Amicosante, Jean Marie Frère, Gian Maria Rossolini

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The location and environment of the acquired bla(IMP) gene, which encodes the IMP-1 metallo-β-lactamase, were investigated in a Japanese Pseudomonas aeruginosa clinical isolate (isolate 101/1477) that produced the enzyme. In this isolate, bla(IMP) was carried on a 36-kb plasmid, and similar to the identical alleles found in Serratia marcescens and Klebsiella pneumoniae clinical isolates, it was located on a mobile gene cassette inserted into an integron. The entire structure of this integron, named In31, was determined. In31 is a class 1 element belonging to the same group of defective transposon derivatives that originated from Tn402-like ancestors such as In0, In2, and In5. The general structure of In31 appeared to be most closely related to that of In5 from pSCH884, suggesting a recent common phylogeny for these two elements. In In31, the bla(IMP) cassette is the first of an array of five gene cassettes that also includes an aacA4 cassette and three original cassettes that have never been described in other integrons. The novel cassettes carry, respectively, (i) a new chloramphenicol acetyltransferase-encoding allele of the catB family, (ii) a qac allele encoding a new member of the small multidrug resistance family of proteins, and (iii) an open reading frame encoding a protein of unknown function. All the resistance genes carried on cassettes inserted in In31 were found to be functional in decreasing the in vitro susceptibilities of host strains to the corresponding antimicrobial agents.

Original languageEnglish
Pages (from-to)890-901
Number of pages12
JournalAntimicrobial Agents and Chemotherapy
Volume43
Issue number4
Publication statusPublished - Apr 1999

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Integrons
Inosine Monophosphate
Pseudomonas aeruginosa
Alleles
Genes
P-Glycoproteins
Serratia marcescens
Chloramphenicol O-Acetyltransferase
Klebsiella pneumoniae
Phylogeny
Anti-Infective Agents
Open Reading Frames
Plasmids
Enzymes
Proteins

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Laraki, N., Galleni, M., Thamm, I., Riccio, M. L., Amicosante, G., Frère, J. M., & Rossolini, G. M. (1999). Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes. Antimicrobial Agents and Chemotherapy, 43(4), 890-901.

Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes. / Laraki, Nezha; Galleni, Moreno; Thamm, Iris; Riccio, Maria Letizia; Amicosante, Gianfranco; Frère, Jean Marie; Rossolini, Gian Maria.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 4, 04.1999, p. 890-901.

Research output: Contribution to journalArticle

Laraki, N, Galleni, M, Thamm, I, Riccio, ML, Amicosante, G, Frère, JM & Rossolini, GM 1999, 'Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes', Antimicrobial Agents and Chemotherapy, vol. 43, no. 4, pp. 890-901.
Laraki, Nezha ; Galleni, Moreno ; Thamm, Iris ; Riccio, Maria Letizia ; Amicosante, Gianfranco ; Frère, Jean Marie ; Rossolini, Gian Maria. / Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 4. pp. 890-901.
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