Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes.

R. Giorda, H. L. Ennis

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-acid sequence. A computer search for homology to known proteins revealed that the 76-amino-acid repeat was identical to human and bovine ubiquitin except for two amino acid differences.

Original languageEnglish
Pages (from-to)2097-2103
Number of pages7
JournalMolecular and Cellular Biology
Volume7
Issue number6
Publication statusPublished - Jun 1987

Fingerprint

Dictyostelium
Ubiquitin
Clone Cells
Amino Acids
Tandem Repeat Sequences
Genes
Amino Acid Sequence
Complementary DNA
Peptides
Genomic Library
Sequence Homology
Germination
Spores
Proteins
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes. / Giorda, R.; Ennis, H. L.

In: Molecular and Cellular Biology, Vol. 7, No. 6, 06.1987, p. 2097-2103.

Research output: Contribution to journalArticle

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