Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes.

R. Giorda, H. L. Ennis

Research output: Contribution to journalArticle

Abstract

A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-acid sequence. A computer search for homology to known proteins revealed that the 76-amino-acid repeat was identical to human and bovine ubiquitin except for two amino acid differences.

Original languageEnglish
Pages (from-to)2097-2103
Number of pages7
JournalMolecular and Cellular Biology
Volume7
Issue number6
Publication statusPublished - Jun 1987

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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