Studies of terminal deoxynucleotidyl transferase in normal and neoplastic human cells.

F. Campagnari, L. Clerici, E. Bombardieri, P. Vezzoni, G. Di Fronzo, M. L. Villa, G. L. Buraggi

Research output: Contribution to journalArticle

Abstract

Optimized biochemical assays and cytoimmunofluorescence tests were used to detect terminal deoxynucleotidyl transferase, TdT, in malignant cells of 36 leukemias and 75 lymphomas from patients not receiving chemotherapy. TdT was virtually absent from normal lymph nodes and from leukocytes of chronic lymphocytic leukemia, CLL, taken as controls. Its quantitative distribution in the neoplasms matched the current knowledge. Appreciable amounts of TdT were found in all the 10 lymphomas of lymphoblastic type, LL, and in the white blood cells of: 16 out of 19 acute lymphoblastic leukemia, AAL, perhaps with modulation in the various phenotypes; 2 out of 3 acute undifferentiated leukemias, AUL; and 3 out of 7 blastic crises in chronic myelogenous leukemia, b.c. CML. Biochemical and cytoimmunological analyses yielded concordant responses and even roughly comparable estimates in the same patients. TdT immunofluorescence was clearly nuclear in most cells and was cytoplasmic occasionally. Definite correlations between concentrations of enzymatic activity and percentage of immunofluorescent cells could not e established. Further detailed work will be required to identify putative subgroups in TdT-positive blast populations.

Original languageEnglish
Pages (from-to)305-320
Number of pages16
JournalAdvances in Experimental Medicine and Biology
Volume145
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Studies of terminal deoxynucleotidyl transferase in normal and neoplastic human cells.'. Together they form a unique fingerprint.

  • Cite this

    Campagnari, F., Clerici, L., Bombardieri, E., Vezzoni, P., Di Fronzo, G., Villa, M. L., & Buraggi, G. L. (1982). Studies of terminal deoxynucleotidyl transferase in normal and neoplastic human cells. Advances in Experimental Medicine and Biology, 145, 305-320.