TY - JOUR
T1 - Studies of the differentiation properties of camptothecin in the human leukaemic cells K562
AU - McSheehy, Paul M J
AU - Gervasoni, Marco
AU - Lampasona, Vito
AU - Erba, Eugenio
AU - D'Incalci, Maurizio
PY - 1991
Y1 - 1991
N2 - Camptothecin, a specific inhibitor of topoisomerase I, caused erythroid differentiation of the human leukaemia cell-line K562, as assessed by benzidine staining at 70 h recovery following a 60 min treatment of the cells. Differentiation was confirmed by increased levels of ε{lunate}-globin and γ-globin mRNA in the treated cells and was accompanied by down-regulation of c-myb mRNA. Synchronisation of K562 cells by non-cytotoxic doses of methotrexate increased the differentiation induced by camptothecin, without affecting the camptothecin-induced inhibition of cellular proliferation. Camptothecin induction of differentiation and inhibition of proliferation may occur by independent mechanisms.
AB - Camptothecin, a specific inhibitor of topoisomerase I, caused erythroid differentiation of the human leukaemia cell-line K562, as assessed by benzidine staining at 70 h recovery following a 60 min treatment of the cells. Differentiation was confirmed by increased levels of ε{lunate}-globin and γ-globin mRNA in the treated cells and was accompanied by down-regulation of c-myb mRNA. Synchronisation of K562 cells by non-cytotoxic doses of methotrexate increased the differentiation induced by camptothecin, without affecting the camptothecin-induced inhibition of cellular proliferation. Camptothecin induction of differentiation and inhibition of proliferation may occur by independent mechanisms.
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U2 - 10.1016/0277-5379(91)90021-5
DO - 10.1016/0277-5379(91)90021-5
M3 - Article
C2 - 1660292
AN - SCOPUS:0025952004
VL - 27
SP - 1406
EP - 1411
JO - European Journal of Cancer
JF - European Journal of Cancer
SN - 0959-8049
IS - 11
ER -