Studies of the differentiation properties of camptothecin in the human leukaemic cells K562

Paul M J McSheehy, Marco Gervasoni, Vito Lampasona, Eugenio Erba, Maurizio D'Incalci

Research output: Contribution to journalArticlepeer-review

Abstract

Camptothecin, a specific inhibitor of topoisomerase I, caused erythroid differentiation of the human leukaemia cell-line K562, as assessed by benzidine staining at 70 h recovery following a 60 min treatment of the cells. Differentiation was confirmed by increased levels of ε{lunate}-globin and γ-globin mRNA in the treated cells and was accompanied by down-regulation of c-myb mRNA. Synchronisation of K562 cells by non-cytotoxic doses of methotrexate increased the differentiation induced by camptothecin, without affecting the camptothecin-induced inhibition of cellular proliferation. Camptothecin induction of differentiation and inhibition of proliferation may occur by independent mechanisms.

Original languageEnglish
Pages (from-to)1406-1411
Number of pages6
JournalEuropean Journal of Cancer and Clinical Oncology
Volume27
Issue number11
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Studies of the differentiation properties of camptothecin in the human leukaemic cells K562'. Together they form a unique fingerprint.

Cite this