Studies of the relationship between cell proliferation and cell death. III. AP-1 DNA-binding activity during concanavalin A-induced proliferation or dexamethasone-induced apoptosis of rat thymocytes

E. Sikora, E. Grassilli, E. Bellesia, L. Troiano, C. Franceschi

Research output: Contribution to journalArticle

Abstract

In previous papers we started to investigate the possible relationship existing between cell proliferation and cell death. We reported that in rat thymocytes, undergoing cell proliferation upon stimulation with Concavalin A or cell death following dexamethasone treatment, an induction of c-fos and c- jun occurs with different but partially overlapping kinetics (Grassilli et al., BBRC, 1992, 188, 1261-1266). To test the hypothesis that, in both processes, the early induction of these nuclear oncogenes is necessary to allow the induction of later-expressed genes regulated at the transcriptional level by AP-1 complex, AP-1 DNA-binding activity was investigated in the aforementioned model. Indeed, we found that AP-1 is activated not only in proliferating but also in apoptotic cells, with a kinetics in accord with our data about c-fos and c-jun mRNA accumulation in the same experimental model. Moreover, our findings suggest that an interaction between AP-1 and glucocorticoid receptor also occurs in our model of dexamethasone-mediated apoptosis.

Original languageEnglish
Pages (from-to)386-391
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number2
DOIs
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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