Studies of the site and distribution of CD34+ cells in idiopathic myelofibrosis

Hongyu Ni, Giovanni Barosi, Damiano Rondelli, Ronald Huffman

Research output: Contribution to journalArticle


The frequency and distribution of CD34+ cells in the bone marrow (BM) of patients with idiopathic myelofibrosis (IM) were determined using an immunohistochemical technique. The percentage and absolute number of circulating CD34+ cells were enumerated. Patients with IM exhibited a continuum of number of BM CD34+ cells ranging from 1 to 85 per 5 mm 2. The frequency of BM CD34+ cells was inversely related to the number of circulating CD34+ cells. The BM biopsy specimens obtained from 4 patients with IM who underwent allogeneic stem cell transplantation were examined sequentially. Quantitative measurement revealed that the reticulin fiber volume was progressively reduced after allogeneic stem cell transplantation. All 4 patients had normocellular marrow with normal numbers of BM CD34+ cells after transplantation. These findings suggest that the BM fibrosis and abnormal hematopoietic stem cell distribution in patients with IM is a consequence of the progeny of a malignant hematopoietic stem cell clone.

Original languageEnglish
Pages (from-to)833-839
Number of pages7
JournalAmerican Journal of Clinical Pathology
Issue number6
Publication statusPublished - Jun 2005


  • Bone marrow
  • CD34+ cells
  • Idiopathic myelofibrosis
  • Myeloproliferative disorders

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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