Studies on allergens of Dermatophagoides pteronyssinus by direct and indirect RAST

S. Romagnani, P. Boccaccini, A. Amadori, M. Ricci

Research output: Contribution to journalArticle

Abstract

Dermatophagoides pteronyssinus (DP) extract was fractionated by Sephadex G 75 and liquid isoelectric focusing (IEF) and the allergenic activity of different fractions was monitored by direct and indirect RAST. The fractionation on Sephadex G 75 showed that the allergenic activity of DP extract was related to wide molecular weight spectrum components, even though the maximum amount was recovered in effluent that contained protein with a molecular weight ranging between 25,000 and 12,500 daltons. By fractionation of the mite extract on IEF, three main peaks of allergenic activity (pI

Original languageEnglish
Pages (from-to)525-535
Number of pages11
JournalInternational Archives of Allergy and Applied Immunology
Volume50
Issue number5
Publication statusPublished - 1976

ASJC Scopus subject areas

  • Immunology and Allergy

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