Studies on DNA damage: Discordant responses of rate of DNA disentanglement (viscosimetrically evaluated) and alkaline elution rate, obtained for several compounds - Possible explanations of the discrepancies

S. Parodi, C. Balbi, M. L. Abelmoschi, M. Pala, P. Russo, L. Santi

Research output: Contribution to journalArticle

Abstract

Alkaline elution is a well-known method for detecting DNA damage. Recently we have developed a viscosimetric method that is even more sensitive than alkaline elution. Here we report that the two methods, although apparently both revealing alkaline DNA fragmentation, can give dramatically different results for a significant series of compounds. We suspect that alkaline elution might reveal not only DNA fragmentation but also the extent of disentanglement of chromatin structure, whereas this DNA disentanglement rate, when evaluated viscosimetrically, is more strictly correlated with the initiation of DNA unwinding.

Original languageEnglish
Pages (from-to)285-300
Number of pages16
JournalCell Biophysics
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 1983

Keywords

  • alkaline elution and DNA damage determination
  • carcinogens, determination of DNA damage by
  • DNA damage, viscometric method for determining
  • DNA viscosity, determination of DNA damage by
  • mutagens, determination of DNA damage by

ASJC Scopus subject areas

  • Cell Biology
  • Biophysics

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