Alkaline elution is a well-known method for detecting DNA damage. Recently we have developed a viscosimetric method that is even more sensitive than alkaline elution. Here we report that the two methods, although apparently both revealing alkaline DNA fragmentation, can give dramatically different results for a significant series of compounds. We suspect that alkaline elution might reveal not only DNA fragmentation but also the extent of disentanglement of chromatin structure, whereas this DNA disentanglement rate, when evaluated viscosimetrically, is more strictly correlated with the initiation of DNA unwinding.
- alkaline elution and DNA damage determination
- carcinogens, determination of DNA damage by
- DNA damage, viscometric method for determining
- DNA viscosity, determination of DNA damage by
- mutagens, determination of DNA damage by
ASJC Scopus subject areas
- Cell Biology