Studies on maitotoxin-induced intracellular Ca2+ elevation in chinese hamster ovary cells stably transfected with cDNAs encoding for L-type Ca2+ channel subunits

Mauro Cataldi, Agnese Secondo, Angela D'Alessio, Maurizio Taglialatela, Franz Hofmann, Norbert Klugbauer, Gianfranco Di Renzo, Lucio Annunziato

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present study was to characterize the role played by different L-type Ca2+ channel subunits in [Ca2+](i) increase induced by maitotoxin (MTX). In the presence of 5 mM extracellular K+, MTX (0.01-0.5 ng/ml) induced a significant concentration-dependent increase in Fura-2- monitored [Ca2+](i) in single Chinese hamster ovary (CHO) cells expressing the α(1c) (CHOCα(9) cells) or the α(1c)β3α2δ (CHOCα9β3α2/δ4 cells) subunits of voltage-gated Ca2+ channels (VGCCs), whereas the effect was much reduced in wild-type CHO cells lacking VGCCs. In addition, MTX effect on CHOCα9, CHOCα9β3α2/δ4, and GH3 cells (0.01-0.1 ng/ml) was inhibited by the selective L-type Ca2+ channel entry-blocker nimodipine (10 μM); a nimodipine-insensitive component was still present, particularly at high (>1 ng/ml) toxin concentrations. In CHOCα9β3α2/δ4 cells, depolarizing concentrations of extracellular K+ (55 mM) reinforced the [Ca2+](i) increase induced by MTX (0.1 ng/ml), and this effect was prevented by nimodipine (10 mM). Finally, patchclamp experiments in CHOCα9β3α2/δ4 cells showed that low MTX concentrations (0.03 ng/ml) induced the occurrence of an inward current at -60 mV, which was completely prevented by Cd2+ (100 μM) and by nimodipine (10 μM), whereas the same dihydropyridine concentration (10 μM) failed to prevent the electrophysiological effects of a higher toxin concentration (3 ng/ml). In conclusion, the results of the present study showed that MTX-induced [Ca2+](i) elevation involves two components: 1) an action on L-type VGCCs at the pore-forming α1c subunit level, which is responsible for the greatest rise of [Ca2+](i); and 2) a VGCC-independent mechanism that is present both in excitable and in nonexcitable cells and is responsible for a lower elevation of [Ca2+](i).

Original languageEnglish
Pages (from-to)725-730
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume290
Issue number2
Publication statusPublished - Aug 1999

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Studies on maitotoxin-induced intracellular Ca2+ elevation in chinese hamster ovary cells stably transfected with cDNAs encoding for L-type Ca2+ channel subunits'. Together they form a unique fingerprint.

Cite this