Several substances interacting with the clotting mechanism of mammalians were tested on lysate of Limulus hemolymphe cells. Gelation was observed in the presence of thrombin, reptilase, botropase, thrombin-coagulase and rabbit or human thromboplastin. Acidification experiments suggested that the above mentioned substances are able to activate, like endotoxin, an inactive proenzyme in the lysate to form an enzyme capable to induce the coagulogen gelation. In contrast, only trypsin would be able to transform directly the coagulogen into a gel.
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine