Studies on the in vitro effects of auto-anti-IgE. Inhibition of total and specific serum IgE detection by a human IgG autoantibody to IgE

R. Paganelli, I. Quinti, G. P. D'Offizi, C. Papetti, R. Nisini, F. Aiuti

Research output: Contribution to journalArticle

Abstract

We isolated a human IgG anti IgE autoantibody by affinity chromatography on a myelomatous IgE (N.D.) coated Sepharose column, from the serum of a patient with atopic eczema, and probed its ability to inhibit IgE recognition by heteroantisera to IgE used in commonly available assays for serum total and specific IgE determinations. (Paper radioimmunosorbent test-PRIST and Phadezym radioallergosorbent test-RAST, both from Pharmacia, Uppsala-Sweden). Our results indicate that IgG anti IgE decrease total IgE results by 10-90% according to its weight ratio to IgE molecules present in samples devoid of endogeneous anti-IgE. Sera with high content of specific IgE to house dust mite (RAST class 3 or 4) are less affected by this autoantibody, but RAST class 2 may be significantly lowered when purified IgG anti IgE is added. These data imply that anti IgE can affect both total and specific IgE determinations.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalJournal of Clinical and Laboratory Immunology
Volume26
Issue number3
Publication statusPublished - 1988

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Autoantibodies
Immunoglobulin E
Immunoglobulin G
Serum
Radioimmunosorbent Test
Radioallergosorbent Test
Pyroglyphidae
In Vitro Techniques
anti-IgE antibodies
Atopic Dermatitis
Affinity Chromatography
Sweden
Sepharose
Weights and Measures

ASJC Scopus subject areas

  • Immunology

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Studies on the in vitro effects of auto-anti-IgE. Inhibition of total and specific serum IgE detection by a human IgG autoantibody to IgE. / Paganelli, R.; Quinti, I.; D'Offizi, G. P.; Papetti, C.; Nisini, R.; Aiuti, F.

In: Journal of Clinical and Laboratory Immunology, Vol. 26, No. 3, 1988, p. 153-157.

Research output: Contribution to journalArticle

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AU - Quinti, I.

AU - D'Offizi, G. P.

AU - Papetti, C.

AU - Nisini, R.

AU - Aiuti, F.

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N2 - We isolated a human IgG anti IgE autoantibody by affinity chromatography on a myelomatous IgE (N.D.) coated Sepharose column, from the serum of a patient with atopic eczema, and probed its ability to inhibit IgE recognition by heteroantisera to IgE used in commonly available assays for serum total and specific IgE determinations. (Paper radioimmunosorbent test-PRIST and Phadezym radioallergosorbent test-RAST, both from Pharmacia, Uppsala-Sweden). Our results indicate that IgG anti IgE decrease total IgE results by 10-90% according to its weight ratio to IgE molecules present in samples devoid of endogeneous anti-IgE. Sera with high content of specific IgE to house dust mite (RAST class 3 or 4) are less affected by this autoantibody, but RAST class 2 may be significantly lowered when purified IgG anti IgE is added. These data imply that anti IgE can affect both total and specific IgE determinations.

AB - We isolated a human IgG anti IgE autoantibody by affinity chromatography on a myelomatous IgE (N.D.) coated Sepharose column, from the serum of a patient with atopic eczema, and probed its ability to inhibit IgE recognition by heteroantisera to IgE used in commonly available assays for serum total and specific IgE determinations. (Paper radioimmunosorbent test-PRIST and Phadezym radioallergosorbent test-RAST, both from Pharmacia, Uppsala-Sweden). Our results indicate that IgG anti IgE decrease total IgE results by 10-90% according to its weight ratio to IgE molecules present in samples devoid of endogeneous anti-IgE. Sera with high content of specific IgE to house dust mite (RAST class 3 or 4) are less affected by this autoantibody, but RAST class 2 may be significantly lowered when purified IgG anti IgE is added. These data imply that anti IgE can affect both total and specific IgE determinations.

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