Studies on the mechanism of interleukin 1 stimulation of platelet activating factor synthesis in human endothelial cells in culture

F. Bussolino, F. Breviario, M. Aglietta, F. Sanavio, A. Bosia, E. Dejana

Research output: Contribution to journalArticle

Abstract

Interleukin 1 promotes the conversion of the biologically inactive lyso-platelet activating factor (lyso-PAF) to the bioactive platelet activating factor (PAF) by an acetylation reaction in cultured human endothelial cells. After 2 h stimulation with interleukin 1, 1-O-alkyl-2-lysoglycero-3-phosphocholine (GPC): acetyl CoA acetyltransferase is activated, reaching a plateau after 6 h and then declining to the basal value within 24 h. This time course is comparable to that of PAF production. These cells are able to incorporate [3H]acetate and [3H]lyso-PAF into PAF. Synthetized [3H]PAF is then catabolized in [3H]alkylacyl phosphoglycerides. 1-O-alkyl-2-acetylglycerol: CDP-choline cholinephosphotransferase and 1-O-alkyl-2-acetyl-GPC: acetyl-hydrolase activities are both present in endothelial cells, but are not activated under our conditions of stimuli. These findings indicate that interleukin 1 induces the PAF synthesis by a deacylation/reacetylation mechanism into human endothelial cells.

Original languageEnglish
Pages (from-to)43-54
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume927
Issue number1
DOIs
Publication statusPublished - Jan 19 1987

Keywords

  • (Human endothelial cell)
  • Interleukin 1
  • PAF, lyso
  • Platelet activating factor

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

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