TY - JOUR
T1 - Studies on the mechanism of metabolic stimulation in polymorphonuclear leucocytes during phagocytosis II. Presence of the NADPH2 oxidizing activity in a myeloperoxidase-deficient subject
AU - Patriarca, P.
AU - Cramer, R.
AU - Tedesco, F.
AU - Kakinuma, K.
PY - 1975/4/7
Y1 - 1975/4/7
N2 - 1. 1. The biochemical properties of leucocytes from a myeloperoxidase-deficient subject were compared with those of leucocytes from healthy subjects. 2. 2. Myeloperoxidase-deficient leucocytes respond to phagocytosis of heat-killed bacteria with increased respiration, increased oxidation of glucose through the hexose monophosphate shunt and increased production of H2O2 as normal leucocytes do. 3. 3. The ability of granules isolated from myeloperoxidase-deficient leucocytes to oxidize nicotinamide coenzymes was comparable to that of granules isolated from normal leucocytes. 4. 4. The results argue against the hypothesis that oxidation of NADPH2 in leucocytes is performed by myeloperoxidase.
AB - 1. 1. The biochemical properties of leucocytes from a myeloperoxidase-deficient subject were compared with those of leucocytes from healthy subjects. 2. 2. Myeloperoxidase-deficient leucocytes respond to phagocytosis of heat-killed bacteria with increased respiration, increased oxidation of glucose through the hexose monophosphate shunt and increased production of H2O2 as normal leucocytes do. 3. 3. The ability of granules isolated from myeloperoxidase-deficient leucocytes to oxidize nicotinamide coenzymes was comparable to that of granules isolated from normal leucocytes. 4. 4. The results argue against the hypothesis that oxidation of NADPH2 in leucocytes is performed by myeloperoxidase.
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U2 - 10.1016/0304-4165(75)90368-2
DO - 10.1016/0304-4165(75)90368-2
M3 - Article
C2 - 236011
AN - SCOPUS:0016805902
VL - 385
SP - 387
EP - 393
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 2
ER -