Studies on the mechanism of metabolic stimulation in polymorphonuclear leucocytes during phagocytosis II. Presence of the NADPH2 oxidizing activity in a myeloperoxidase-deficient subject

P. Patriarca, R. Cramer, F. Tedesco, K. Kakinuma

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The biochemical properties of leucocytes from a myeloperoxidase-deficient subject were compared with those of leucocytes from healthy subjects. 2. 2. Myeloperoxidase-deficient leucocytes respond to phagocytosis of heat-killed bacteria with increased respiration, increased oxidation of glucose through the hexose monophosphate shunt and increased production of H2O2 as normal leucocytes do. 3. 3. The ability of granules isolated from myeloperoxidase-deficient leucocytes to oxidize nicotinamide coenzymes was comparable to that of granules isolated from normal leucocytes. 4. 4. The results argue against the hypothesis that oxidation of NADPH2 in leucocytes is performed by myeloperoxidase.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalBBA - General Subjects
Volume385
Issue number2
DOIs
Publication statusPublished - Apr 7 1975

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

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