Studies on the oncogenic potential of Epstein-Barr-virus (EBV)-infected B cells in AIDS-related disorders

S. Roncella, P. Caretto, G. Forni, G. Cutrona, A. Verde, D. Ramarli, P. F. Di Celle, R. Foa, M. Sessarego, V. Pistoia, M. Ferrarini

Research output: Contribution to journalArticlepeer-review

Abstract

Spontaneous lymphoblastoid cell lines (LCLs) were established from the peripheral blood of 10 human immunodeficiency virus (HIV)-seropositive patients in order to investigate whether or not progression of the cells towards a malignant state could be traced. The LCLs studied displayed no differences in their surface phenotype, karyotype, and tumorigenicity in nude mice as compared with a wide panel of control LCLs. Furthermore, no c-myc rearrangement could be detected in any of the LCLs. However, 4 of the 10 LCLs derived from HIV-seropositive patients formed colonies in agar with a cloning efficiency of 0.1-0.9%. This percentage was much lower than that of a control neoplastic B cell line (50%), but consistently higher than that observed for a battery of spontaneous LCLs. The cells of a number of sublines that were derived from the agar colonies expressed new activation markers (CD10 and Bac-1) but did not induce tumors in nude mice or display chromosomal abnormalities. These sublines might comprise cells that have progressed towards a more markedly transformed state.

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalInternational Journal of Cancer
Issue numberSUPPL. 4
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)
  • Oncology

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