Studies on the relationship between cell proliferation and cell death: Opposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in pha-stimulated human lymphocytes

E. Grassilli, S. Bettuzzi, D. Monti, M. C. Ingletti, C. Franceschi, A. Corti

Research output: Contribution to journalArticle

Abstract

To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemoagglutinin (PHA). ODC is the rate limiting enzyme of polyamines biosynthesis and its early induction in mitogen-stimulated lymphocytes has been reported. On the other hand, SGP-2, a glycoprotein present in most mammalian tissues, is induced in classical models of apoptosis, such as dexamethasone-treated thymocytes. Indeed, a consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHAstimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number1
DOIs
Publication statusPublished - Oct 15 1991

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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