Studies on the relationship between cell proliferation and cell death: Opposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in pha-stimulated human lymphocytes

E. Grassilli, S. Bettuzzi, D. Monti, M. C. Ingletti, C. Franceschi, A. Corti

Research output: Contribution to journalArticle

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Abstract

To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemoagglutinin (PHA). ODC is the rate limiting enzyme of polyamines biosynthesis and its early induction in mitogen-stimulated lymphocytes has been reported. On the other hand, SGP-2, a glycoprotein present in most mammalian tissues, is induced in classical models of apoptosis, such as dexamethasone-treated thymocytes. Indeed, a consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHAstimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number1
DOIs
Publication statusPublished - Oct 15 1991

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Clusterin
Ornithine Decarboxylase
Lymphocytes
Cell proliferation
Cell death
Cell Death
Cell Proliferation
Messenger RNA
Blood
Biosynthesis
Polyamines
Thymocytes
Mitogens
Dexamethasone
Glycoproteins
Cell Cycle
Genes
Cells
Tissue
Apoptosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Studies on the relationship between cell proliferation and cell death : Opposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in pha-stimulated human lymphocytes. / Grassilli, E.; Bettuzzi, S.; Monti, D.; Ingletti, M. C.; Franceschi, C.; Corti, A.

In: Biochemical and Biophysical Research Communications, Vol. 180, No. 1, 15.10.1991, p. 59-63.

Research output: Contribution to journalArticle

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