Studies on trypsin activation of inactive renin in rat plasma

N. Glorioso, P. Madeddu, P. Dessi-Fulgheri, V. Anania, F. Meloni, G. Fois, F. Bandiera, A. Rappelli

Research output: Contribution to journalArticle

Abstract

The experimental conditions of trypsin activation of inactive renin in rat plasma have been studied. Our results showed that the pH optimum of trypsin-activated renin is 6.2, the same as that of rat plasma active renin. Trypsin concentration and incubation time might also interfere with the activation process. Trypsin concentration below 2 mg/ml cannot activate inactive renin. Trypsin at a concentration of 6 mg/ml for 1 min. can cleave dialyzable fragments from renin substrate which can generate Angiotensin I at 37°C with a pH optimum of 5.3 but which do not affect Angiotensin generation at pH 6.2. Longer incubations (more than 2 min.), on the contrary, can produce a cleavage at 4°C of Angiotensin I containing fragments directly from renin substrate strongly interfering with measurements of renin activity at pH 6.2. Trypsin concentrations lower than endogenous rat plasma anti-trypsin activity do not activate inactive renin. Much higher concentrations of trypsin, however, are needed to obtain optimum activation of inactive renin. in average, 40% of the total circulating renin in rat plasma can be activated by trypsin.

Original languageEnglish
Pages (from-to)2203-2212
Number of pages10
JournalClinical and Experimental Hypertension
VolumeA4
Issue number11-12
DOIs
Publication statusPublished - 1982

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Renin
Trypsin
Angiotensin I
Angiotensinogen
Angiotensins

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology

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Glorioso, N., Madeddu, P., Dessi-Fulgheri, P., Anania, V., Meloni, F., Fois, G., ... Rappelli, A. (1982). Studies on trypsin activation of inactive renin in rat plasma. Clinical and Experimental Hypertension, A4(11-12), 2203-2212. https://doi.org/10.3109/10641968209062384

Studies on trypsin activation of inactive renin in rat plasma. / Glorioso, N.; Madeddu, P.; Dessi-Fulgheri, P.; Anania, V.; Meloni, F.; Fois, G.; Bandiera, F.; Rappelli, A.

In: Clinical and Experimental Hypertension, Vol. A4, No. 11-12, 1982, p. 2203-2212.

Research output: Contribution to journalArticle

Glorioso, N, Madeddu, P, Dessi-Fulgheri, P, Anania, V, Meloni, F, Fois, G, Bandiera, F & Rappelli, A 1982, 'Studies on trypsin activation of inactive renin in rat plasma', Clinical and Experimental Hypertension, vol. A4, no. 11-12, pp. 2203-2212. https://doi.org/10.3109/10641968209062384
Glorioso, N. ; Madeddu, P. ; Dessi-Fulgheri, P. ; Anania, V. ; Meloni, F. ; Fois, G. ; Bandiera, F. ; Rappelli, A. / Studies on trypsin activation of inactive renin in rat plasma. In: Clinical and Experimental Hypertension. 1982 ; Vol. A4, No. 11-12. pp. 2203-2212.
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