Study of a soluble tumor-associated marker composed of CEA related molecules recognized by three monoclonal antibodies

A. Mastroianni, E. Tagliabue, F. Centis, R. Pellegrini, S. Martignone, S. Menard, M. I. Colnaghi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three MAbs, MLuC2, MLuC8 and MLuC9, directed against a molecule that is produced and secreted by carcinoma cells were studied with the aim of developing a double-determinant immunoradiometric assay (DDIRMA). We demonstrated by means of immunoblotting, immunodepletion and DDIRMA techniques, that MLuC9 reacted against the CEA molecule, whereas MLuC2 and MLuC8 reacted against a 90 Kd molecule related to CEA. The DDIRMA performed with the anti-CEA as a catcher MAb and the anti-90 Kd as a tracer MAb was found to be positive with the HT29 soluble extract, which suggests the existence of CEA/90 Kd dimeric molecules. The same reactivity was found when sera from patients with lung carcinomas were tested, which excludes that this molecule could be an artefact due to the cell solubilization procedures. The association between CEA and the 90 Kd molecule was further confirmed by immunodepletion experiments in which the immunoprecipitation with one MAb not only removed the recognized molecule, but also partially immunodepleted the material from the other.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalInternational Journal of Biological Markers
Volume7
Issue number1
Publication statusPublished - 1992

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Immunoradiometric Assay
Tumor Biomarkers
Tumors
Monoclonal Antibodies
Molecules
Carcinoma
Assays
Immunoprecipitation
Immunoblotting
Artifacts
Lung
Serum
Cells
Association reactions

ASJC Scopus subject areas

  • Biochemistry
  • Immunology

Cite this

Study of a soluble tumor-associated marker composed of CEA related molecules recognized by three monoclonal antibodies. / Mastroianni, A.; Tagliabue, E.; Centis, F.; Pellegrini, R.; Martignone, S.; Menard, S.; Colnaghi, M. I.

In: International Journal of Biological Markers, Vol. 7, No. 1, 1992, p. 21-26.

Research output: Contribution to journalArticle

Mastroianni, A, Tagliabue, E, Centis, F, Pellegrini, R, Martignone, S, Menard, S & Colnaghi, MI 1992, 'Study of a soluble tumor-associated marker composed of CEA related molecules recognized by three monoclonal antibodies', International Journal of Biological Markers, vol. 7, no. 1, pp. 21-26.
Mastroianni, A. ; Tagliabue, E. ; Centis, F. ; Pellegrini, R. ; Martignone, S. ; Menard, S. ; Colnaghi, M. I. / Study of a soluble tumor-associated marker composed of CEA related molecules recognized by three monoclonal antibodies. In: International Journal of Biological Markers. 1992 ; Vol. 7, No. 1. pp. 21-26.
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AU - Centis, F.

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AU - Martignone, S.

AU - Menard, S.

AU - Colnaghi, M. I.

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