Study of chromatographic profiles of hGH in normal and acromegalic subjects

F. Minuto, A. Barreca, S. Ferrini

Research output: Contribution to journalArticle

Abstract

The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.

Original languageEnglish
Pages (from-to)740-741
Number of pages2
JournalIRCS Medical Science
Volume11
Issue number8
Publication statusPublished - 1983

Fingerprint

Somatomedins
Blood
Phlebotomy
Centrifugation
Fetal Blood
Forearm
Growth Hormone
Radioimmunoassay
Gel Chromatography
Veins
Gels
Scanning electron microscopy
Temperature
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Minuto, F., Barreca, A., & Ferrini, S. (1983). Study of chromatographic profiles of hGH in normal and acromegalic subjects. IRCS Medical Science, 11(8), 740-741.

Study of chromatographic profiles of hGH in normal and acromegalic subjects. / Minuto, F.; Barreca, A.; Ferrini, S.

In: IRCS Medical Science, Vol. 11, No. 8, 1983, p. 740-741.

Research output: Contribution to journalArticle

Minuto, F, Barreca, A & Ferrini, S 1983, 'Study of chromatographic profiles of hGH in normal and acromegalic subjects', IRCS Medical Science, vol. 11, no. 8, pp. 740-741.
Minuto, F. ; Barreca, A. ; Ferrini, S. / Study of chromatographic profiles of hGH in normal and acromegalic subjects. In: IRCS Medical Science. 1983 ; Vol. 11, No. 8. pp. 740-741.
@article{4b1fa3d10cc14ba5abf3facea8afa73c,
title = "Study of chromatographic profiles of hGH in normal and acromegalic subjects",
abstract = "The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90{\%}), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46{\%}) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83{\%}). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95{\%}), 6.7 + 1.8 ng.ml (17.15 + 4.81{\%}) and 26.82 + 3.62 ng/ml (68.5 + 3.92{\%}). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30{\%} of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.",
author = "F. Minuto and A. Barreca and S. Ferrini",
year = "1983",
language = "English",
volume = "11",
pages = "740--741",
journal = "IRCS Medical Science",
issn = "0305-6651",
publisher = "MTP International Research Communications ltd.",
number = "8",

}

TY - JOUR

T1 - Study of chromatographic profiles of hGH in normal and acromegalic subjects

AU - Minuto, F.

AU - Barreca, A.

AU - Ferrini, S.

PY - 1983

Y1 - 1983

N2 - The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.

AB - The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.

UR - http://www.scopus.com/inward/record.url?scp=0020566761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020566761&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0020566761

VL - 11

SP - 740

EP - 741

JO - IRCS Medical Science

JF - IRCS Medical Science

SN - 0305-6651

IS - 8

ER -