Abstract
The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.
| Original language | English |
|---|---|
| Pages (from-to) | 740-741 |
| Number of pages | 2 |
| Journal | IRCS Medical Science |
| Volume | 11 |
| Issue number | 8 |
| Publication status | Published - 1983 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Study of chromatographic profiles of hGH in normal and acromegalic subjects. / Minuto, F.; Barreca, A.; Ferrini, S.
In: IRCS Medical Science, Vol. 11, No. 8, 1983, p. 740-741.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Study of chromatographic profiles of hGH in normal and acromegalic subjects
AU - Minuto, F.
AU - Barreca, A.
AU - Ferrini, S.
PY - 1983
Y1 - 1983
N2 - The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.
AB - The chromatographic pattern of circulating growth hormone was studied by Spehadex G-100 gel-filtration in 6 acromegalic subjects and in 4 newborns. Blood was obtained by venipuncture of a forearm vein in acromegalics and from cord blood, immediately after delivery, in newborns. Serum was obtained by centrifugation after clotting at room temperature and stored frozen (-20° C) until chromatographed. GH was assayed in every fraction by radioimmunoassay. Big-big GH was in acromegalics 18.7 ± 6.7 ng/ml (M + SEM, 19.02 ± 2.90%), Big-GH was 20.4 + 12.6 ng/ml (16.53 + 6.46%) and little was 50.7 + 12.7 ng/ml (64.44 + 5.83%). In newborns the three forms were respectively 5.43 + 0.9 ng/ml (14.43 + 2.95%), 6.7 + 1.8 ng.ml (17.15 + 4.81%) and 26.82 + 3.62 ng/ml (68.5 + 3.92%). No statistically significant difference was noticed between the two groups studied. Although the monomeric form is predominant it should be noted that the macromolecular forms are quite abundant (about 30% of total circulating GH) and, therefore, must have some pathophysiological significance. The fact that newborn subjects show similar profiles, however, is in contrast with the hypothesis that the polymeric forms are due to biosynthetic alterations of the adenomatous cells. The opposite biological effect of GH hypersecretion in these two groups (high somatomedin in acromegalics, low somatomedin in newborns) therefore seems to be due mainly to a different responsiveness of the somatomedin-inducer system.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020566761&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0020566761
VL - 11
SP - 740
EP - 741
JO - IRCS Medical Science
JF - IRCS Medical Science
SN - 0305-6651
IS - 8
ER -