Abstract
A detailed investigation of the factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives (i.e. cysteine, homocysteine and cysteinylglycine) is described. Essentially, this assay entails extracting specific thiols by plasma disulphide bond reduction, protein precipitation, sulphydryl compound derivatization with the thiol-specific fluorogenic reagent ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F), and subsequent separation with isocratic reversed-phase high-performance liquid chromatography. By improving the reliability of several analytical parameters (composition of the mobile phase, pretreatment of the sample using different reducing and protein precipitation agents, and optimization of the derivatization of thiols with SBD-F), a number of critical issues can be identified and solved.
| Original language | English |
|---|---|
| Pages (from-to) | 209-215 |
| Number of pages | 7 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 706 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 20 1998 |
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Keywords
- 7-Fluorobenzo-2-oxa-1,3-diazole-4-sulfonate
- Amino acids
- Cysteine
- Cysteinylglycine
- Derivatization
- Homocysteine
- LC
- Thiols
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Study of factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives by liquid chromatography. / Rizzo, V.; Montalbetti, L.; Valli, M.; Bosoni, T.; Scoglio, E.; Moratti, R.
In: Journal of Chromatography B: Biomedical Applications, Vol. 706, No. 2, 20.03.1998, p. 209-215.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Study of factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives by liquid chromatography
AU - Rizzo, V.
AU - Montalbetti, L.
AU - Valli, M.
AU - Bosoni, T.
AU - Scoglio, E.
AU - Moratti, R.
PY - 1998/3/20
Y1 - 1998/3/20
N2 - A detailed investigation of the factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives (i.e. cysteine, homocysteine and cysteinylglycine) is described. Essentially, this assay entails extracting specific thiols by plasma disulphide bond reduction, protein precipitation, sulphydryl compound derivatization with the thiol-specific fluorogenic reagent ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F), and subsequent separation with isocratic reversed-phase high-performance liquid chromatography. By improving the reliability of several analytical parameters (composition of the mobile phase, pretreatment of the sample using different reducing and protein precipitation agents, and optimization of the derivatization of thiols with SBD-F), a number of critical issues can be identified and solved.
AB - A detailed investigation of the factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives (i.e. cysteine, homocysteine and cysteinylglycine) is described. Essentially, this assay entails extracting specific thiols by plasma disulphide bond reduction, protein precipitation, sulphydryl compound derivatization with the thiol-specific fluorogenic reagent ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F), and subsequent separation with isocratic reversed-phase high-performance liquid chromatography. By improving the reliability of several analytical parameters (composition of the mobile phase, pretreatment of the sample using different reducing and protein precipitation agents, and optimization of the derivatization of thiols with SBD-F), a number of critical issues can be identified and solved.
KW - 7-Fluorobenzo-2-oxa-1,3-diazole-4-sulfonate
KW - Amino acids
KW - Cysteine
KW - Cysteinylglycine
KW - Derivatization
KW - Homocysteine
KW - LC
KW - Thiols
UR - http://www.scopus.com/inward/record.url?scp=0032549680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032549680&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00547-1
DO - 10.1016/S0378-4347(97)00547-1
M3 - Article
C2 - 9551807
AN - SCOPUS:0032549680
VL - 706
SP - 209
EP - 215
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 2
ER -