Abstract
In a previous work it has been demonstrated that micro-Raman spectroscopy is a technique able to recognize crystalline phases on untreated samples. In that case, inorganic particles and uncoated fibers from bronchoalveolar lavage (BAL) of a patient affected by pneumoconiosis were identified and characterized. In this work the technique is applied to asbestos bodies, that is, to coated fibers, and on crystallizations and fibrous phases observed in the plural plaque from patients affected by mesothelioma. From the Raman analysis the abundant fibrous material observed in the pleural area is talc, whereas rounded grains in the pleural tissue show the Raman spectrum of apatite, a calcium phosphate mineral particular to bones. In the pulmonary tissue many asbestos bodies, consisting of the incorporated fibers coated by iron-rich proteins, were observed. Under the 632.8 nm laser beam of the spectrometer, photocrystallization of hematite in the iron-rich material forming the asbestos bodies can be proposed by the changes in the Raman spectra acquired during subsequent acquisitions. Nevertheless, the identification of the mineral phase constituting the incorporated fiber was possible by analyzing the Raman spectra; the results were confirmed by variable pressure scanning electron microscopy with annexed energy dispersive spectroscopy (VP-SEM-EDS) analyses.
| Original language | English |
|---|---|
| Pages (from-to) | 571-577 |
| Number of pages | 7 |
| Journal | Applied Spectroscopy |
| Volume | 64 |
| Issue number | 6 |
| Publication status | Published - Jun 2010 |
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Keywords
- Asbestos bodies
- EDS
- Energy dispersive spectroscopy
- Inorganic particles
- Micro-Raman spectroscopy
- SEM
- Talc pleurodesis
- Variable pressure scanning electron microscopy
ASJC Scopus subject areas
- Spectroscopy
- Instrumentation
- Medicine(all)
Cite this
Study of inorganic particles, fibers, and asbestos bodies by variable pressure scanning electron microscopy with annexed energy dispersive spectroscopy and micro-raman spectroscopy in thin sections of lung and pleural plaque. / Rinaudo, Caterina; Croce, Alessandro; Musa, Maya; Fornero, Elisa; Allegrina, Mario; Trivero, Paolo; Bellis, Donata; Sferch, Daniela; Toffalorio, Francesca; Veronesi, Giulia; Pelosi, Giuseppe.
In: Applied Spectroscopy, Vol. 64, No. 6, 06.2010, p. 571-577.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Study of inorganic particles, fibers, and asbestos bodies by variable pressure scanning electron microscopy with annexed energy dispersive spectroscopy and micro-raman spectroscopy in thin sections of lung and pleural plaque
AU - Rinaudo, Caterina
AU - Croce, Alessandro
AU - Musa, Maya
AU - Fornero, Elisa
AU - Allegrina, Mario
AU - Trivero, Paolo
AU - Bellis, Donata
AU - Sferch, Daniela
AU - Toffalorio, Francesca
AU - Veronesi, Giulia
AU - Pelosi, Giuseppe
PY - 2010/6
Y1 - 2010/6
N2 - In a previous work it has been demonstrated that micro-Raman spectroscopy is a technique able to recognize crystalline phases on untreated samples. In that case, inorganic particles and uncoated fibers from bronchoalveolar lavage (BAL) of a patient affected by pneumoconiosis were identified and characterized. In this work the technique is applied to asbestos bodies, that is, to coated fibers, and on crystallizations and fibrous phases observed in the plural plaque from patients affected by mesothelioma. From the Raman analysis the abundant fibrous material observed in the pleural area is talc, whereas rounded grains in the pleural tissue show the Raman spectrum of apatite, a calcium phosphate mineral particular to bones. In the pulmonary tissue many asbestos bodies, consisting of the incorporated fibers coated by iron-rich proteins, were observed. Under the 632.8 nm laser beam of the spectrometer, photocrystallization of hematite in the iron-rich material forming the asbestos bodies can be proposed by the changes in the Raman spectra acquired during subsequent acquisitions. Nevertheless, the identification of the mineral phase constituting the incorporated fiber was possible by analyzing the Raman spectra; the results were confirmed by variable pressure scanning electron microscopy with annexed energy dispersive spectroscopy (VP-SEM-EDS) analyses.
AB - In a previous work it has been demonstrated that micro-Raman spectroscopy is a technique able to recognize crystalline phases on untreated samples. In that case, inorganic particles and uncoated fibers from bronchoalveolar lavage (BAL) of a patient affected by pneumoconiosis were identified and characterized. In this work the technique is applied to asbestos bodies, that is, to coated fibers, and on crystallizations and fibrous phases observed in the plural plaque from patients affected by mesothelioma. From the Raman analysis the abundant fibrous material observed in the pleural area is talc, whereas rounded grains in the pleural tissue show the Raman spectrum of apatite, a calcium phosphate mineral particular to bones. In the pulmonary tissue many asbestos bodies, consisting of the incorporated fibers coated by iron-rich proteins, were observed. Under the 632.8 nm laser beam of the spectrometer, photocrystallization of hematite in the iron-rich material forming the asbestos bodies can be proposed by the changes in the Raman spectra acquired during subsequent acquisitions. Nevertheless, the identification of the mineral phase constituting the incorporated fiber was possible by analyzing the Raman spectra; the results were confirmed by variable pressure scanning electron microscopy with annexed energy dispersive spectroscopy (VP-SEM-EDS) analyses.
KW - Asbestos bodies
KW - EDS
KW - Energy dispersive spectroscopy
KW - Inorganic particles
KW - Micro-Raman spectroscopy
KW - SEM
KW - Talc pleurodesis
KW - Variable pressure scanning electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=77953839784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953839784&partnerID=8YFLogxK
M3 - Article
C2 - 20537223
AN - SCOPUS:77953839784
VL - 64
SP - 571
EP - 577
JO - Applied Spectroscopy
JF - Applied Spectroscopy
SN - 0003-7028
IS - 6
ER -