Study of T-cell activation in Type I diabetic patients and pre-Type I diabetic subjects by cytometric analysis: Antigen expression defect in vitro

C. Giordano, R. de Maria, M. Todaro, G. Stassi, A. Mattina, P. Richiusa, G. Galluzzo, F. Pantó, A. Galluzzo

Research output: Contribution to journalArticle

Abstract

In Type I diabetes the observation of a decreased release of interleukin-2 (IL-2) and soluble IL-2 receptors by means of stimulated lymphocytes in vitro indicates that a primary immunoregulatory defect may be involved. To confirm this hypothesis we investigated the T-cell activation trend, evaluating the surface expression of IL-2 receptor (CD25), transferrin (CD71), HLA class II (DR), and CD69 phenotypes after in vitro stimulation with phytohemagglutinin (PHA; 1 and 10 μg/ml) and concanavalin A (12.5 μg/ml) in six newly diagnosed Type I diabetics and six islet cell- and insulin autoantibody-positive first-degree relatives. As controls were studied six long-standing Type I diabetics and six healthy subjects. T-cell cultures from the four groups were performed on the same day and examined at 0, 24, 48, 96, 120, and 144 hr. Cytometric analysis was performed, keeping PBMC gating constant on the basis of physical parameters (scatter and volume). Using both PHA concentrations, a lower level of CD25, CD71, CD69, and DR antigen expression was found in newly diagnosed patients at all observation times with respect to control cultures (P

Original languageEnglish
Pages (from-to)68-78
Number of pages11
JournalJournal of Clinical Immunology
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1993

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Keywords

  • CD25 cells
  • CD69 cells
  • CD71 cells
  • DR cells
  • flow cytometry
  • interleukin-2 secretion defect
  • pre-Type I diabetes
  • T-cell activation
  • T-cell cultures
  • Type I diabetes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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