Study of tryptophan metabolism via serotonin in cerebrospinal fluid of patients with noncommunicating hydrocephalus using a new endoscopic technique

Stefano Comai, Pierluigi Longatti, Alessandro Perin, Antonella Bertazzo, Eugenio Ragazzi, C. V L Costa, Graziella Allegri

Research output: Contribution to journalArticle

Abstract

By a recent minimally invasive neuroendoscopic technique, the cerebral ventricles have been reached in a quick, reliable, and harmless way, making possible the study of cerebrospinal fluid (CSF) of the lateral ventricles and, above all, the CSF adjacent to the walls of the third ventricle. Tryptophan, 5-hydroxytryptophan, serotonin (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) were measured in CSF by HPLC equipment. Twenty-six patients affected with noncommunicating hydrocephalus were enrolled in the study and, as controls, 28 subjects not suffering from any neurological disease. The concentrations of tryptophan were higher in right ventricular CSF than in lumbar CSF (P <0.01). 5-HT was detectable in the CSF of the right ventricle of hydrocephalic patients. 5-HIAA was higher in right ventricular CSF than in cisternal and lumbar CSF (P <0.01), both in controls and in hydrocephalic patients. However, there was a higher concentration of 5-HIAA in right ventricular (P <0.05) and cisternal (P <0.01) CSF in hydrocephalic patients in comparison with controls. In the CSF samples withdrawn during neuroendoscopy, 5-HT presented the highest concentrations in the pineal recess. The highest amounts of 5-HIAA were found in the choroid plexus, third and right ventricles, pituitary recess, and aqueduct, and the lowest in pineal recess, subarachnoid space, infundibulum, and interpeduncolar cistern. These results provide new insight into the fate of tryptophan and its metabolites via serotonin in the CSF and suggest the feasibility of the new neuroendoscopic technique for brain metabolic studies.

Original languageEnglish
Pages (from-to)683-691
Number of pages9
JournalJournal of Neuroscience Research
Volume84
Issue number3
DOIs
Publication statusPublished - Aug 15 2006

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Hydrocephalus
Tryptophan
Cerebrospinal Fluid
Serotonin
Hydroxyindoleacetic Acid
5-Hydroxytryptophan
Third Ventricle
Heart Ventricles
Neuroendoscopy
Cerebral Ventricles
Subarachnoid Space
Choroid Plexus
Lateral Ventricles
Pituitary Gland
High Pressure Liquid Chromatography
Equipment and Supplies

Keywords

  • CSF
  • Hydrocephalus
  • Neuroendoscopy
  • Serotonin
  • Tryptophan

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Study of tryptophan metabolism via serotonin in cerebrospinal fluid of patients with noncommunicating hydrocephalus using a new endoscopic technique. / Comai, Stefano; Longatti, Pierluigi; Perin, Alessandro; Bertazzo, Antonella; Ragazzi, Eugenio; Costa, C. V L; Allegri, Graziella.

In: Journal of Neuroscience Research, Vol. 84, No. 3, 15.08.2006, p. 683-691.

Research output: Contribution to journalArticle

Comai, Stefano ; Longatti, Pierluigi ; Perin, Alessandro ; Bertazzo, Antonella ; Ragazzi, Eugenio ; Costa, C. V L ; Allegri, Graziella. / Study of tryptophan metabolism via serotonin in cerebrospinal fluid of patients with noncommunicating hydrocephalus using a new endoscopic technique. In: Journal of Neuroscience Research. 2006 ; Vol. 84, No. 3. pp. 683-691.
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