Study on the Association among Mycotoxins and other Variables in Children with Autism

Barbara De Santis, Maria Elisabetta Raggi, Giorgio Moretti, Francesco Facchiano, Alessandra Mezzelani, Laura Villa, Arianna Bonfanti, Alessandra Campioni, Stefania Rossi, Serena Camposeo, Sabina Soricelli, Gabriele Moracci, Francesca Debegnach, Emanuela Gregori, Francesca Ciceri, Luciano Milanesi, Anna Marabotti, Carlo Brera

Research output: Contribution to journalArticle

Abstract

Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n= 172) and a group of controls (n= 61) (siblings and non-parental) were recruited in North and South Italy. All children had blood and urine samples taken, for testing some mycotoxins by a LC-MS/MS validated method. Blood samples were also tested for assessing specific IgG against food and fungal antigens and cytokines. The analyses outputs highlighted statistically significant differences comparing mycotoxins levels between (i) children groups both in urine (deoxynivalenol and de-epoxydeoxynivalenol,p= 0.0141 andp= 0.0259, respectively) and serum (aflatoxin M1, ochratoxin A and fumonisin B1,p= 0.0072,p= 0.0141 andp= 0.0061, respectively); (ii) a group of selected fungal IgGs, and IgGs against wheat and gluten and (iii) cytokines. These results suggest the need for a deeper examination of the role that mycotoxins may have on the etiology of ASD.

Original languageEnglish
JournalToxins
Volume9
Issue number7
DOIs
Publication statusPublished - Jun 29 2017

Fingerprint

Mycotoxins
Autistic Disorder
Blood
Fungal Antigens
Aflatoxin M1
Urine
Cytokines
Food
Glutens
Genetic Predisposition to Disease
Italy
Triticum
Deterioration
Siblings
Cross-Sectional Studies
Immunoglobulin G
Health
Impurities
Control Groups
Testing

Keywords

  • Antibodies, Fungal/immunology
  • Antigens, Fungal/immunology
  • Autism Spectrum Disorder/blood
  • Child
  • Child, Preschool
  • Cytokines/blood
  • Environmental Exposure/analysis
  • Female
  • Glutens/immunology
  • Humans
  • Immunoglobulin G/immunology
  • Male
  • Mycotoxins/blood
  • Triticum/immunology

Cite this

Study on the Association among Mycotoxins and other Variables in Children with Autism. / De Santis, Barbara; Raggi, Maria Elisabetta; Moretti, Giorgio; Facchiano, Francesco; Mezzelani, Alessandra; Villa, Laura; Bonfanti, Arianna; Campioni, Alessandra; Rossi, Stefania; Camposeo, Serena; Soricelli, Sabina; Moracci, Gabriele; Debegnach, Francesca; Gregori, Emanuela; Ciceri, Francesca; Milanesi, Luciano; Marabotti, Anna; Brera, Carlo.

In: Toxins, Vol. 9, No. 7, 29.06.2017.

Research output: Contribution to journalArticle

De Santis, B, Raggi, ME, Moretti, G, Facchiano, F, Mezzelani, A, Villa, L, Bonfanti, A, Campioni, A, Rossi, S, Camposeo, S, Soricelli, S, Moracci, G, Debegnach, F, Gregori, E, Ciceri, F, Milanesi, L, Marabotti, A & Brera, C 2017, 'Study on the Association among Mycotoxins and other Variables in Children with Autism', Toxins, vol. 9, no. 7. https://doi.org/10.3390/toxins9070203
De Santis, Barbara ; Raggi, Maria Elisabetta ; Moretti, Giorgio ; Facchiano, Francesco ; Mezzelani, Alessandra ; Villa, Laura ; Bonfanti, Arianna ; Campioni, Alessandra ; Rossi, Stefania ; Camposeo, Serena ; Soricelli, Sabina ; Moracci, Gabriele ; Debegnach, Francesca ; Gregori, Emanuela ; Ciceri, Francesca ; Milanesi, Luciano ; Marabotti, Anna ; Brera, Carlo. / Study on the Association among Mycotoxins and other Variables in Children with Autism. In: Toxins. 2017 ; Vol. 9, No. 7.
@article{7b6fd2ac35274d548b23519054cb1a33,
title = "Study on the Association among Mycotoxins and other Variables in Children with Autism",
abstract = "Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n= 172) and a group of controls (n= 61) (siblings and non-parental) were recruited in North and South Italy. All children had blood and urine samples taken, for testing some mycotoxins by a LC-MS/MS validated method. Blood samples were also tested for assessing specific IgG against food and fungal antigens and cytokines. The analyses outputs highlighted statistically significant differences comparing mycotoxins levels between (i) children groups both in urine (deoxynivalenol and de-epoxydeoxynivalenol,p= 0.0141 andp= 0.0259, respectively) and serum (aflatoxin M1, ochratoxin A and fumonisin B1,p= 0.0072,p= 0.0141 andp= 0.0061, respectively); (ii) a group of selected fungal IgGs, and IgGs against wheat and gluten and (iii) cytokines. These results suggest the need for a deeper examination of the role that mycotoxins may have on the etiology of ASD.",
keywords = "Antibodies, Fungal/immunology, Antigens, Fungal/immunology, Autism Spectrum Disorder/blood, Child, Child, Preschool, Cytokines/blood, Environmental Exposure/analysis, Female, Glutens/immunology, Humans, Immunoglobulin G/immunology, Male, Mycotoxins/blood, Triticum/immunology",
author = "{De Santis}, Barbara and Raggi, {Maria Elisabetta} and Giorgio Moretti and Francesco Facchiano and Alessandra Mezzelani and Laura Villa and Arianna Bonfanti and Alessandra Campioni and Stefania Rossi and Serena Camposeo and Sabina Soricelli and Gabriele Moracci and Francesca Debegnach and Emanuela Gregori and Francesca Ciceri and Luciano Milanesi and Anna Marabotti and Carlo Brera",
year = "2017",
month = "6",
day = "29",
doi = "10.3390/toxins9070203",
language = "English",
volume = "9",
journal = "Toxins",
issn = "2072-6651",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "7",

}

TY - JOUR

T1 - Study on the Association among Mycotoxins and other Variables in Children with Autism

AU - De Santis, Barbara

AU - Raggi, Maria Elisabetta

AU - Moretti, Giorgio

AU - Facchiano, Francesco

AU - Mezzelani, Alessandra

AU - Villa, Laura

AU - Bonfanti, Arianna

AU - Campioni, Alessandra

AU - Rossi, Stefania

AU - Camposeo, Serena

AU - Soricelli, Sabina

AU - Moracci, Gabriele

AU - Debegnach, Francesca

AU - Gregori, Emanuela

AU - Ciceri, Francesca

AU - Milanesi, Luciano

AU - Marabotti, Anna

AU - Brera, Carlo

PY - 2017/6/29

Y1 - 2017/6/29

N2 - Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n= 172) and a group of controls (n= 61) (siblings and non-parental) were recruited in North and South Italy. All children had blood and urine samples taken, for testing some mycotoxins by a LC-MS/MS validated method. Blood samples were also tested for assessing specific IgG against food and fungal antigens and cytokines. The analyses outputs highlighted statistically significant differences comparing mycotoxins levels between (i) children groups both in urine (deoxynivalenol and de-epoxydeoxynivalenol,p= 0.0141 andp= 0.0259, respectively) and serum (aflatoxin M1, ochratoxin A and fumonisin B1,p= 0.0072,p= 0.0141 andp= 0.0061, respectively); (ii) a group of selected fungal IgGs, and IgGs against wheat and gluten and (iii) cytokines. These results suggest the need for a deeper examination of the role that mycotoxins may have on the etiology of ASD.

AB - Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n= 172) and a group of controls (n= 61) (siblings and non-parental) were recruited in North and South Italy. All children had blood and urine samples taken, for testing some mycotoxins by a LC-MS/MS validated method. Blood samples were also tested for assessing specific IgG against food and fungal antigens and cytokines. The analyses outputs highlighted statistically significant differences comparing mycotoxins levels between (i) children groups both in urine (deoxynivalenol and de-epoxydeoxynivalenol,p= 0.0141 andp= 0.0259, respectively) and serum (aflatoxin M1, ochratoxin A and fumonisin B1,p= 0.0072,p= 0.0141 andp= 0.0061, respectively); (ii) a group of selected fungal IgGs, and IgGs against wheat and gluten and (iii) cytokines. These results suggest the need for a deeper examination of the role that mycotoxins may have on the etiology of ASD.

KW - Antibodies, Fungal/immunology

KW - Antigens, Fungal/immunology

KW - Autism Spectrum Disorder/blood

KW - Child

KW - Child, Preschool

KW - Cytokines/blood

KW - Environmental Exposure/analysis

KW - Female

KW - Glutens/immunology

KW - Humans

KW - Immunoglobulin G/immunology

KW - Male

KW - Mycotoxins/blood

KW - Triticum/immunology

U2 - 10.3390/toxins9070203

DO - 10.3390/toxins9070203

M3 - Article

C2 - 28661468

VL - 9

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 7

ER -