Studying nonobstructive azoospermia in cystinosis: Histologic examination of testes and epididymis and sperm analysis in a Ctns -/- mouse model

Martine T P Besouw, Ans M M Van Pelt, Héloïse P. Gaide Chevronnay, Pierre J. Courtoy, Anna Pastore, Ellen Goossens, Olivier Devuyst, Corinne Antignac, Elena N. Levtchenko

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns -/- mouse model. Design: Observational case-control study. Setting: Academic research laboratory. Animal(s): Male C57BL/6 Ctns -/- mice were compared with C57BL/6 wild-type (wt) mice. Intervention(s): None. Main Outcome Measure(s): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3). Result(s): Mean testicular cystine content was significantly higher in Ctns -/- mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns -/- mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found. Conclusion(s): The Ctns -/- mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.

Original languageEnglish
Pages (from-to)162-165
Number of pages4
JournalFertility and Sterility
Volume98
Issue number1
DOIs
Publication statusPublished - Jul 2012

Keywords

  • CASA
  • Cystinosis
  • epididymis
  • infertility
  • nonobstructive azoospermia
  • sperm analysis
  • testis

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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