Styrene-catabolism regulation in Pseudomonas fluorescens ST

Phosphorylation of StyR induces dimerization and cooperative DNA-binding

Livia Leoni, Paolo Ascenzi, Alessio Bocedi, Giordano Rampioni, Laura Castellini, Elisabetta Zennaro

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Styrene is an important chemical extensively used in the petrochemical and polymer industries. In Pseudomonas fluorescens ST, styrene metabolism is controlled by a two-component regulatory system, very uncommon in the degradation of aromatic compounds. The two-component regulatory proteins StyS and StyR regulate the expression of the styABCD operon, which codes for styrene degradation. StyS corresponds to the sensor kinase and StyR to the response regulator, which is essential for the activation of PstyA, the promoter of the catabolic operon. In two-component systems, the response regulator is phosphorylated by the cognate sensor kinase. Phosphorylation activates the response regulator, inducing DNA-binding. The mechanism underlying this activation has been reported only for a very few response regulators. Here, the effect of phosphorylation on the oligomeric state and on the DNA-binding properties of StyR has been investigated. Phosphorylation induces dimerization of StyR, the affinity of dimeric StyR for the target DNA is higher than that of the monomer, moreover dimeric StyR binding to the DNA target is cooperative. Furthermore, StyR oligomerization may be driven by the DNA target. This is the first direct demonstration that StyR response regulator binds to the PstyA promoter.

Original languageEnglish
Pages (from-to)926-931
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number3
DOIs
Publication statusPublished - Apr 11 2003

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Pseudomonas fluorescens
Phosphorylation
Styrene
Dimerization
DNA
Operon
Phosphotransferases
Chemical activation
Degradation
Oligomerization
Aromatic compounds
Sensors
Metabolism
Petrochemicals
Industry
Polymers
Demonstrations
Monomers
Proteins

Keywords

  • Pseudomonas fluorescens ST
  • Response regulator oligomerization
  • Response regulator phosphorylation
  • StyR response regulator
  • Styrene
  • Two-component regulatory system

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Styrene-catabolism regulation in Pseudomonas fluorescens ST : Phosphorylation of StyR induces dimerization and cooperative DNA-binding. / Leoni, Livia; Ascenzi, Paolo; Bocedi, Alessio; Rampioni, Giordano; Castellini, Laura; Zennaro, Elisabetta.

In: Biochemical and Biophysical Research Communications, Vol. 303, No. 3, 11.04.2003, p. 926-931.

Research output: Contribution to journalArticle

Leoni, Livia ; Ascenzi, Paolo ; Bocedi, Alessio ; Rampioni, Giordano ; Castellini, Laura ; Zennaro, Elisabetta. / Styrene-catabolism regulation in Pseudomonas fluorescens ST : Phosphorylation of StyR induces dimerization and cooperative DNA-binding. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 303, No. 3. pp. 926-931.
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