Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST

Francesco Massai, Giordano Rampioni, Chiara Micolonghi, Marco Messina, Elisabetta Zennaro, Paolo Ascenzi, Livia Leoni

Research output: Contribution to journalArticle

Abstract

In Pseudomonas fluorescens ST, the expression of the pathway for the degradation of the toxic compound styrene is induced by the substrate (i.e., styrene) and repressed by alternative carbon sources. This regulation relies on a two-component system consisting of the sensor kinase StyS and the response regulator StyR. StyS belongs to the group of hybrid “double sensor kinases”, a unique family of sensor kinases having two ATP catalytic domains, each one preceded by a PAS domain. Here, the styrene-based phosphorylation of StyS is reported. Styrene is specifically sensed by the StyS N-terminal PAS sensor domain, enhancing the StyS phosphorylation level.

Original languageEnglish
Pages (from-to)1177-1182
Number of pages6
JournalAnnals of Microbiology
Volume65
Issue number2
DOIs
Publication statusPublished - Jul 11 2014

Fingerprint

Pseudomonas fluorescens
Styrene
Phosphotransferases
Bacteria
Phosphorylation
Poisons
Catalytic Domain
Carbon
Adenosine Triphosphate

Keywords

  • Bacterial two-component systems
  • Degradation of aromatic pollutants
  • Histidine kinase
  • Pseudomonas fluorescens ST
  • Styrene

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST. / Massai, Francesco; Rampioni, Giordano; Micolonghi, Chiara; Messina, Marco; Zennaro, Elisabetta; Ascenzi, Paolo; Leoni, Livia.

In: Annals of Microbiology, Vol. 65, No. 2, 11.07.2014, p. 1177-1182.

Research output: Contribution to journalArticle

Massai, Francesco ; Rampioni, Giordano ; Micolonghi, Chiara ; Messina, Marco ; Zennaro, Elisabetta ; Ascenzi, Paolo ; Leoni, Livia. / Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST. In: Annals of Microbiology. 2014 ; Vol. 65, No. 2. pp. 1177-1182.
@article{80c8e877385e4b8aa41b31a1b985f7af,
title = "Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST",
abstract = "In Pseudomonas fluorescens ST, the expression of the pathway for the degradation of the toxic compound styrene is induced by the substrate (i.e., styrene) and repressed by alternative carbon sources. This regulation relies on a two-component system consisting of the sensor kinase StyS and the response regulator StyR. StyS belongs to the group of hybrid “double sensor kinases”, a unique family of sensor kinases having two ATP catalytic domains, each one preceded by a PAS domain. Here, the styrene-based phosphorylation of StyS is reported. Styrene is specifically sensed by the StyS N-terminal PAS sensor domain, enhancing the StyS phosphorylation level.",
keywords = "Bacterial two-component systems, Degradation of aromatic pollutants, Histidine kinase, Pseudomonas fluorescens ST, Styrene",
author = "Francesco Massai and Giordano Rampioni and Chiara Micolonghi and Marco Messina and Elisabetta Zennaro and Paolo Ascenzi and Livia Leoni",
year = "2014",
month = "7",
day = "11",
doi = "10.1007/s13213-014-0931-y",
language = "English",
volume = "65",
pages = "1177--1182",
journal = "Annals of Microbiology",
issn = "1590-4261",
publisher = "Springer Heidelberg",
number = "2",

}

TY - JOUR

T1 - Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST

AU - Massai, Francesco

AU - Rampioni, Giordano

AU - Micolonghi, Chiara

AU - Messina, Marco

AU - Zennaro, Elisabetta

AU - Ascenzi, Paolo

AU - Leoni, Livia

PY - 2014/7/11

Y1 - 2014/7/11

N2 - In Pseudomonas fluorescens ST, the expression of the pathway for the degradation of the toxic compound styrene is induced by the substrate (i.e., styrene) and repressed by alternative carbon sources. This regulation relies on a two-component system consisting of the sensor kinase StyS and the response regulator StyR. StyS belongs to the group of hybrid “double sensor kinases”, a unique family of sensor kinases having two ATP catalytic domains, each one preceded by a PAS domain. Here, the styrene-based phosphorylation of StyS is reported. Styrene is specifically sensed by the StyS N-terminal PAS sensor domain, enhancing the StyS phosphorylation level.

AB - In Pseudomonas fluorescens ST, the expression of the pathway for the degradation of the toxic compound styrene is induced by the substrate (i.e., styrene) and repressed by alternative carbon sources. This regulation relies on a two-component system consisting of the sensor kinase StyS and the response regulator StyR. StyS belongs to the group of hybrid “double sensor kinases”, a unique family of sensor kinases having two ATP catalytic domains, each one preceded by a PAS domain. Here, the styrene-based phosphorylation of StyS is reported. Styrene is specifically sensed by the StyS N-terminal PAS sensor domain, enhancing the StyS phosphorylation level.

KW - Bacterial two-component systems

KW - Degradation of aromatic pollutants

KW - Histidine kinase

KW - Pseudomonas fluorescens ST

KW - Styrene

UR - http://www.scopus.com/inward/record.url?scp=84929843717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929843717&partnerID=8YFLogxK

U2 - 10.1007/s13213-014-0931-y

DO - 10.1007/s13213-014-0931-y

M3 - Article

AN - SCOPUS:84929843717

VL - 65

SP - 1177

EP - 1182

JO - Annals of Microbiology

JF - Annals of Microbiology

SN - 1590-4261

IS - 2

ER -