Subcellular localization of tetanus neurotoxin-insensitive vesicle- associated membrane protein (VAMP)/VAMP7 in neuronal cells: Evidence for a novel membrane compartment

Silvia Coco, Graca Raposo, Sonia Martinez, Jean Jacques Fontaine, Shigeo Takamori, Ahmed Zahraoui, Reinhard Jahn, Michela Matteoli, Daniel Louvard, Thierry Galli

Research output: Contribution to journalArticlepeer-review

Abstract

The clostridial neurotoxin-insensitive soluble N-ethylmaleimide- sensitive factor attachment protein (SNAP) receptors, tetanus neurotoxin- insensitive (TI)-vesicle-associated membrane protein (VAMP)/VAMP7, SNAP23, and syntaxin 3 have recently been implicated in transport of exocytotic vesicles to the apical plasma membrane of epithelial cells. This pathway had been shown previously to be insensitive to tetanus neurotoxin and botulinum neurotoxin F. TI-VAMP/VAMP7 is also a good candidate to be implicated in an exocytotic pathway involved in neurite outgrowth because tetanus neurotoxin does not inhibit this process in conditions in which it abolishes neurotransmitter release. We have now found that TI-VAMP/VAMP7 has a widespread distribution in the adult rat brain in which its localization strikingly differs from that of nerve terminal markers. TI-VAMP/VAMP7 does not enrich in synaptic vesicles nor in large dense-core granules but is associated with light membranes. In hippocampal neurons developing in vitro, TI-VAMP/VAMP7 localizes to vesicles in the axonal and dendritic outgrowths and concentrates into the leading edge of the growth cone, a region devoid of synaptobrevin 2, before synaptogenesis. After the onset of synaptogenesis, TI-VAMP/VAMP7 is found predominantly in the somatodendritic domain. In PC12 cells, TI-VAMP/VAMP7 does not colocalize with synaptobrevin 2, chromogranin B, or several markers of endocytic compartments. At the electron microscopic level, TI-VAMP/VAMP7 is mainly associated with tubules and vesicles. Altogether, these results suggest that TI-VAMP/VAMP7 defines a novel membrane compartment in neurite outgrowths and in the somatodendritic domain.

Original languageEnglish
Pages (from-to)9803-9812
Number of pages10
JournalJournal of Neuroscience
Volume19
Issue number22
Publication statusPublished - Nov 15 1999

Keywords

  • Clostridial neurotoxin
  • Exocytosis
  • Neurite outgrowth
  • SNARE
  • Synaptobrevin 2
  • TI- VAMP/VAMP7

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Subcellular localization of tetanus neurotoxin-insensitive vesicle- associated membrane protein (VAMP)/VAMP7 in neuronal cells: Evidence for a novel membrane compartment'. Together they form a unique fingerprint.

Cite this