Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1681-1688 |
| Number of pages | 8 |
| Journal | Journal of Cellular Physiology |
| Volume | 232 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2017 |
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Keywords
- 16S sequencing
- anaerobes
- idiopathic infertility
- ion torrent
- microbiome
Cite this
Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility. / Campisciano, Giuseppina; Florian, Fiorella; D'Eustacchio, Angela; Stanković, David; Ricci, Giuseppe; Ricci, Giuseppe; De Seta, Francesco; De Seta, Francesco; Comar, Manola; Comar, Manola.
In: Journal of Cellular Physiology, Vol. 232, No. 7, 01.07.2017, p. 1681-1688.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility
AU - Campisciano, Giuseppina
AU - Florian, Fiorella
AU - D'Eustacchio, Angela
AU - Stanković, David
AU - Ricci, Giuseppe
AU - Ricci, Giuseppe
AU - De Seta, Francesco
AU - De Seta, Francesco
AU - Comar, Manola
AU - Comar, Manola
PY - 2017/7/1
Y1 - 2017/7/1
N2 - © 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.
AB - © 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.
KW - 16S sequencing
KW - anaerobes
KW - idiopathic infertility
KW - ion torrent
KW - microbiome
UR - http://www.scopus.com/inward/record.url?scp=85013374224&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013374224&partnerID=8YFLogxK
U2 - 10.1002/jcp.25806
DO - 10.1002/jcp.25806
M3 - Article
C2 - 28098358
AN - SCOPUS:85013374224
VL - 232
SP - 1681
EP - 1688
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 1097-4652
IS - 7
ER -