Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

© 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.
Original languageEnglish
Pages (from-to)1681-1688
Number of pages8
JournalJournal of Cellular Physiology
Volume232
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Microbiota
Infertility
Bacterial Vaginosis
Ribosomal DNA
Biomarkers
Databases
Ions
Therapeutics
Research

Keywords

  • 16S sequencing
  • anaerobes
  • idiopathic infertility
  • ion torrent
  • microbiome

Cite this

@article{8011772201ff45fd85df31de07cc7211,
title = "Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility",
abstract = "{\circledC} 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.",
keywords = "16S sequencing, anaerobes, idiopathic infertility, ion torrent, microbiome",
author = "Giuseppina Campisciano and Fiorella Florian and Angela D'Eustacchio and David Stanković and Giuseppe Ricci and Giuseppe Ricci and {De Seta}, Francesco and {De Seta}, Francesco and Manola Comar and Manola Comar",
year = "2017",
month = "7",
day = "1",
doi = "10.1002/jcp.25806",
language = "English",
volume = "232",
pages = "1681--1688",
journal = "Journal of Cellular Physiology",
issn = "1097-4652",
publisher = "Wiley-Liss Inc.",
number = "7",

}

TY - JOUR

T1 - Subclinical alteration of the cervical–vaginal microbiome in women with idiopathic infertility

AU - Campisciano, Giuseppina

AU - Florian, Fiorella

AU - D'Eustacchio, Angela

AU - Stanković, David

AU - Ricci, Giuseppe

AU - Ricci, Giuseppe

AU - De Seta, Francesco

AU - De Seta, Francesco

AU - Comar, Manola

AU - Comar, Manola

PY - 2017/7/1

Y1 - 2017/7/1

N2 - © 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.

AB - © 2017 Wiley Periodicals, Inc. Biomarkers have a wide application in research and clinic, they help to choose the correct treatment for diseases. Recent studies, addressing the vaginal microbiome using next generation sequencing (NGS), reported the involvement of bacterial species in infertility. We compared the vaginal microbiome of idiopathic infertile women with that of healthy, including bacterial vaginosis affected women and non-idiopathic infertile women, to identify bacterial species suitable as biomarkers. Information on microorganisms was obtained from the V3-16S rDNA sequencing of cervical–vaginal fluids of 96 women using the Ion Torrent platform. Data were processed with QIIME and classified against the Vaginal 16S rDNA Reference Database. The analysis revealed a significant beta-diversity variation (p < 0.001) between the four groups included in the study. L. iners, L. crispatus, and L. gasseri distinguished idiopathic infertile women from the other groups. In these women, a microbial profile similar to that observed in bacterial vaginosis women has been detected. Our results suggest that the quantitative assessment and identification of specific microorganisms of the cervical–vaginal microflora could increase the accuracy of available tools for the diagnosis of infertility and improve the adoption of therapeutic protocols.

KW - 16S sequencing

KW - anaerobes

KW - idiopathic infertility

KW - ion torrent

KW - microbiome

UR - http://www.scopus.com/inward/record.url?scp=85013374224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013374224&partnerID=8YFLogxK

U2 - 10.1002/jcp.25806

DO - 10.1002/jcp.25806

M3 - Article

VL - 232

SP - 1681

EP - 1688

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 1097-4652

IS - 7

ER -