Subfractionation of human very low density lipoproteins by heparin-Sepharose affinity chromatography

E. Trezzi, C. Calvi, P. Roma, A. L. Catapano

Research output: Contribution to journalArticlepeer-review

Abstract

Very low density lipoproteins obtained from normolipidemic subjects were fractionated into subclasses by means of affinity chromatography on a heparin-Sepharose column in the presence of MnCl2. The four subfractions eluted at 0.05, 0.12, 0.20, and 0.38 M NaCl and they differed in chemical composition and apoprotein pattern. The relative amounts of apoB and apoE in subfractions increased with increasing concentration of the NaCl eluant. Modification of the arginyl residues with 1-2 cyclohexadione demonstrated that arginine plays an important role in determining the elution pattern of VLDL. In vitro studies indicated that only fractions eluted at 0.2 and 0.5 M NaCl compete with LDL for cellular receptors. These data suggest that the various subfractions may represent VLDL at different stages of catabolism.

Original languageEnglish
Pages (from-to)790-795
Number of pages6
JournalJournal of Lipid Research
Volume24
Issue number6
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Endocrinology

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