Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy

Philip de Guzman, Yuji Inaba, Giuseppe Biagini, Enrica Baldelli, Cristiana Mollinari, Daniela Merlo, Massimo Avoli

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we used in vitro electrophysiology along with immunohistochemistry and molecular techniques to study the subiculum - a limbic structure that gates the information flow from and to the hippocampus - in pilocarpine-treated epileptic rats. Comparative data were obtained from age-matched nonepileptic controls (NEC). Subicular neurons in hippocampal-entorhinal cortex (EC) slices of epileptic rats were: (i) hyperexcitable when activated by CA1 or EC inputs; and (ii) generated spontaneous postsynaptic potentials at higher frequencies than NEC cells. Analysis of pharmacologically isolated, GABAA receptor-mediated inhibitory postsynaptic potentials revealed more positive reversal potentials in epileptic tissue (-67.8 ± 6.3 mV, n = 16 vs. -74.8 ± 3.6 mV in NEC, n = 13; P <0.001) combined with a reduction in peak conductance (17.6 ± 11.3 nS vs. 41.1 ± 26.7 nS in NEC; P <0.003). These electrophysiological data correlated in the epileptic subiculum with (i) reduced levels of mRNA expression and immunoreactivity of the neuron-specific potassium-chloride cotransporter 2; (ii) decreased number of parvalbumin-positive cells; and (iii) increased synaptophysin (a putative marker of sprouting) immunoreactivity. These findings identify an increase in network excitability within the subiculum of pilocarpine-treated, epileptic rats and point at a reduction in inhibition as an underlying mechanism.

Original languageEnglish
Pages (from-to)843-860
Number of pages18
JournalHippocampus
Volume16
Issue number10
DOIs
Publication statusPublished - 2006

Keywords

  • GABA
  • Parvalbumin
  • Pilocarpine
  • Potassium-chloride contransporter 2
  • Subiculum
  • Temporal lobe epilepsy

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy'. Together they form a unique fingerprint.

Cite this