Substoichiometric concentrations of ATP-G-actin are required to anneal actin polymerized by calcium ions

Enrico Grazi, Giorgio Trombetta, Luciana Rizzieri, Massimo Guidoboni

Research output: Contribution to journalArticlepeer-review

Abstract

At 3°C and pH 7.0, the addition of 40 nM ATP-G-actin to F-actin (12 μM as the monomer), polymerized in the presence of 4 mM CaCl2, determines a substantial and rapid increase of the viscosity of the solution, which is accompanied by the incorporation of the ATP-G-actin added into the polymer. The hypothesis that the presence of ATP-actin at the filament end(s) promotes the annealing reaction is substantiated by the finding that, after the addition of ATP-G-actin, the average filament length is increased. This finding is relevant, not only because it provides evidences in favour of the existence of annealing but also because it shows that the concentration of ATP-G-actin influences the filaments length distribution through a mechanism different from the elongation reaction.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number1
DOIs
Publication statusPublished - Feb 28 1989

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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