Substoichiometric concentrations of ATP-G-actin are required to anneal actin polymerized by calcium ions

Enrico Grazi, Giorgio Trombetta, Luciana Rizzieri, Massimo Guidoboni

Research output: Contribution to journalArticle

Abstract

At 3°C and pH 7.0, the addition of 40 nM ATP-G-actin to F-actin (12 μM as the monomer), polymerized in the presence of 4 mM CaCl2, determines a substantial and rapid increase of the viscosity of the solution, which is accompanied by the incorporation of the ATP-G-actin added into the polymer. The hypothesis that the presence of ATP-actin at the filament end(s) promotes the annealing reaction is substantiated by the finding that, after the addition of ATP-G-actin, the average filament length is increased. This finding is relevant, not only because it provides evidences in favour of the existence of annealing but also because it shows that the concentration of ATP-G-actin influences the filaments length distribution through a mechanism different from the elongation reaction.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number1
DOIs
Publication statusPublished - Feb 28 1989

Fingerprint

Actins
Ions
Calcium
Actin Cytoskeleton
Annealing
Viscosity
Elongation
Polymers
Monomers
ATP-G-actin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Substoichiometric concentrations of ATP-G-actin are required to anneal actin polymerized by calcium ions. / Grazi, Enrico; Trombetta, Giorgio; Rizzieri, Luciana; Guidoboni, Massimo.

In: Biochemical and Biophysical Research Communications, Vol. 159, No. 1, 28.02.1989, p. 7-13.

Research output: Contribution to journalArticle

@article{0606c85145ae456ea6a1c7423ba41544,
title = "Substoichiometric concentrations of ATP-G-actin are required to anneal actin polymerized by calcium ions",
abstract = "At 3°C and pH 7.0, the addition of 40 nM ATP-G-actin to F-actin (12 μM as the monomer), polymerized in the presence of 4 mM CaCl2, determines a substantial and rapid increase of the viscosity of the solution, which is accompanied by the incorporation of the ATP-G-actin added into the polymer. The hypothesis that the presence of ATP-actin at the filament end(s) promotes the annealing reaction is substantiated by the finding that, after the addition of ATP-G-actin, the average filament length is increased. This finding is relevant, not only because it provides evidences in favour of the existence of annealing but also because it shows that the concentration of ATP-G-actin influences the filaments length distribution through a mechanism different from the elongation reaction.",
author = "Enrico Grazi and Giorgio Trombetta and Luciana Rizzieri and Massimo Guidoboni",
year = "1989",
month = "2",
day = "28",
doi = "10.1016/0006-291X(89)92396-6",
language = "English",
volume = "159",
pages = "7--13",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Substoichiometric concentrations of ATP-G-actin are required to anneal actin polymerized by calcium ions

AU - Grazi, Enrico

AU - Trombetta, Giorgio

AU - Rizzieri, Luciana

AU - Guidoboni, Massimo

PY - 1989/2/28

Y1 - 1989/2/28

N2 - At 3°C and pH 7.0, the addition of 40 nM ATP-G-actin to F-actin (12 μM as the monomer), polymerized in the presence of 4 mM CaCl2, determines a substantial and rapid increase of the viscosity of the solution, which is accompanied by the incorporation of the ATP-G-actin added into the polymer. The hypothesis that the presence of ATP-actin at the filament end(s) promotes the annealing reaction is substantiated by the finding that, after the addition of ATP-G-actin, the average filament length is increased. This finding is relevant, not only because it provides evidences in favour of the existence of annealing but also because it shows that the concentration of ATP-G-actin influences the filaments length distribution through a mechanism different from the elongation reaction.

AB - At 3°C and pH 7.0, the addition of 40 nM ATP-G-actin to F-actin (12 μM as the monomer), polymerized in the presence of 4 mM CaCl2, determines a substantial and rapid increase of the viscosity of the solution, which is accompanied by the incorporation of the ATP-G-actin added into the polymer. The hypothesis that the presence of ATP-actin at the filament end(s) promotes the annealing reaction is substantiated by the finding that, after the addition of ATP-G-actin, the average filament length is increased. This finding is relevant, not only because it provides evidences in favour of the existence of annealing but also because it shows that the concentration of ATP-G-actin influences the filaments length distribution through a mechanism different from the elongation reaction.

UR - http://www.scopus.com/inward/record.url?scp=0024516542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024516542&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(89)92396-6

DO - 10.1016/0006-291X(89)92396-6

M3 - Article

C2 - 2923625

AN - SCOPUS:0024516542

VL - 159

SP - 7

EP - 13

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -