Substrate-stimulated oxygen consumption by isolated rat brain microvessels in the presence and absence of ATP

Alberto Villacara, Luigi Garbin, Giuseppe Lauria, Giorgio Zanchin, Lauro Galzigna

Research output: Contribution to journalArticlepeer-review

Abstract

We report here properties of isolated brain microvessels such as the rate of oxygen consumption with different substrates; the permeabilizing effect of added ATP is studied. With the isolation procedure presented the cerebral endothelium has a metabolic activity comparable to that reported in the literature. The respiratory rate of the microvessels is not affected by the addition of ATP, whereas it is significantly increased by addition of succinate and α-chetoglutarate. The exposure of the isolated brain capillaries to ATP, in a Ca2+-free medium, increases the uptake of 6-carboxyfluorescein. This may be due to pores opened by ATP in the endothelial cell membrane in the absence of divalent cations.

Original languageEnglish
Pages (from-to)1113-1116
Number of pages4
JournalNeurochemical Research
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 1993

Keywords

  • ATP
  • brain microvessels
  • oxygen consumption

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

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