Abstract
The large extracellular loop of human CD81, a tetraspanin mediating hepatitis C virus envelope protein E2 binding to human cells, has been crystallized in a hexagonal form. The three-dimensional structure, solved and refined at 2.6 Å resolution (R-factor = 22.8%), shows that the protein adopts a dimeric assembly, based on an association interface built up by tetraspanin-conserved residues. Structural comparisons with the tertiary structure of human CD81 large extracellular loop, previously determined in a different crystal form, show marked conformational fluctuations in the molecular regions thought to be involved in binding to the viral protein, suggesting rules for recognition and assembly within the tetraspan web.
Original language | English |
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Pages (from-to) | 1447-1452 |
Number of pages | 6 |
Journal | Biological Chemistry |
Volume | 383 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sep 1 2002 |
Keywords
- CD81
- HCV E2 glycoprotein
- Hepatitis C virus
- Receptor protein
- Tetraspanins
ASJC Scopus subject areas
- Biochemistry