Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver

Laurent Humeau, Christian Chabannon, Meri T. Firpo, Patrice Mannoni, Claude Bagnis, Maria Grazia Roncarolo, Reiko Namikawa

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Highly purified CD34++CD38-Lin- hematopoietic progenitors isolated from human fetal liver were infected with the murine retroviral vector, MFG nls-LacZ, which encodes a modified version of the Escherichia coli β- galactosidase gene. Progenitors that were cocultured with the packaging cell line could reconstitute human bone marrow or thymus implanted in SCID-hu mice. Expression of the β-galactosidase gene was observed in primitive and committed clonogenic progenitors, mature myeloid, B-lineage cells, and T- lineage cells for up to 4 months after injection into SCID-hu mice. Furthermore, hematopoietic reconstitution by genetically modified progenitor cells could be achieved by the injection of the cells generated from as few as 500 CD34++CD38-Lin- cells, suggesting efficient retroviral gene transfer into fetal liver progenitors.

Original languageEnglish
Pages (from-to)3496-3506
Number of pages11
JournalBlood
Volume90
Issue number9
Publication statusPublished - 1997

Fingerprint

Galactosidases
SCID Mice
Hematopoiesis
Liver
Genes
Gene transfer
Thymus
Escherichia coli
Packaging
Bone
Injections
Cells
Product Packaging
Cell Lineage
Thymus Gland
Stem Cells
Bone Marrow
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Hematology

Cite this

Humeau, L., Chabannon, C., Firpo, M. T., Mannoni, P., Bagnis, C., Roncarolo, M. G., & Namikawa, R. (1997). Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver. Blood, 90(9), 3496-3506.

Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver. / Humeau, Laurent; Chabannon, Christian; Firpo, Meri T.; Mannoni, Patrice; Bagnis, Claude; Roncarolo, Maria Grazia; Namikawa, Reiko.

In: Blood, Vol. 90, No. 9, 1997, p. 3496-3506.

Research output: Contribution to journalArticle

Humeau, L, Chabannon, C, Firpo, MT, Mannoni, P, Bagnis, C, Roncarolo, MG & Namikawa, R 1997, 'Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver', Blood, vol. 90, no. 9, pp. 3496-3506.
Humeau, Laurent ; Chabannon, Christian ; Firpo, Meri T. ; Mannoni, Patrice ; Bagnis, Claude ; Roncarolo, Maria Grazia ; Namikawa, Reiko. / Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver. In: Blood. 1997 ; Vol. 90, No. 9. pp. 3496-3506.
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