Successful reconstitution of human hematopoiesis in the SCID-hu mouse by genetically modified, highly enriched progenitors isolated from fetal liver

Laurent Humeau, Christian Chabannon, Meri T. Firpo, Patrice Mannoni, Claude Bagnis, Maria Grazia Roncarolo, Reiko Namikawa

Research output: Contribution to journalArticlepeer-review

Abstract

Highly purified CD34++CD38-Lin- hematopoietic progenitors isolated from human fetal liver were infected with the murine retroviral vector, MFG nls-LacZ, which encodes a modified version of the Escherichia coli β- galactosidase gene. Progenitors that were cocultured with the packaging cell line could reconstitute human bone marrow or thymus implanted in SCID-hu mice. Expression of the β-galactosidase gene was observed in primitive and committed clonogenic progenitors, mature myeloid, B-lineage cells, and T- lineage cells for up to 4 months after injection into SCID-hu mice. Furthermore, hematopoietic reconstitution by genetically modified progenitor cells could be achieved by the injection of the cells generated from as few as 500 CD34++CD38-Lin- cells, suggesting efficient retroviral gene transfer into fetal liver progenitors.

Original languageEnglish
Pages (from-to)3496-3506
Number of pages11
JournalBlood
Volume90
Issue number9
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Hematology

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