Suggested role of the golgi apparatus and endoplasmic reticulum for crucial sites of hepatitis C virus replication in human lymphoblastoid cells infected in vitro

Annalucia Serafino, Maria Beatrice Valli, Federica Andreola, Annalisa Crema, Giampietro Ravagnan, Luisa Bertolini, Guido Carloni

Research output: Contribution to journalArticle

Abstract

Iacovacci et al. [(1997a) Research in Virology 148:147-151] described that the euploid diploid cells, of the normal human bone marrow-derived lymphoblastoid B-cell line TO.FE., are susceptible to hepatitis C virus (HCV) infection and support long term virus production. Transmission electron microscopy described some steps of HCV replication cycle in this in vitro infected cellular system [Serafino et al. (1997) Research in Virology 148:153-159]. In the present study, in order to identify the intracellular sites involved in HCV replication, the ultrastructural changes associated with infection in TO.FE. cells were correlated with the subcellular localisation of structural and nonstructural viral proteins. Transmission electron microscopy and confocal microscopy data indicate that these viral proteins appeared located in the Golgi apparatus and endoplasmic reticulum, suggesting an active involvement of these compartments in viral assembly and morphogenesis. Furthermore, transmission and scanning electron microscopic observations on cultures infected chronically support the hypothesis that these cellular compartments may serve as starting sites of the morphological changes associated to viral infection and replication, leading to cell-cell fusion, syncytia formation, and finally lysis of infected cells and virus release.

Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalJournal of Medical Virology
Volume70
Issue number1
DOIs
Publication statusPublished - May 1 2003

Keywords

  • B-cell line
  • Confocal microscopy
  • Cytoplasmic compartments
  • Electron microscopy
  • HCV

ASJC Scopus subject areas

  • Virology

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