1H-NMR metabolomic profiling of the crayfish Astacus leptodactylus subjected to polyphenol-enriched diets

Susan Costantini, Lucia Parrillo, Eliana Guerriero, Dominique Melck, Giovanni Colonna, Maria Grazia Volpe, Marina Paolucci

Research output: Contribution to journalArticle

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Abstract

1H-NMR analysis of the hepatopancreas, muscle and haemolymph of Astacus leptodactylus after feeding with polyphenol-enriched diet is reported. 1H-NMR spectra of lipophilic extracts showed the presence of cholesterol, fatty acid residues, phospholipids and triglycerides. 1H-NMR spectra of aqueous extracts identified 35 metabolites in the hepatopancreas, 31 in the muscle and 22 in the haemolymph. A total of 20 metabolites (amino acids and their derivatives) were present in the hepatopancreas, the muscle and the haemolymph. A total of 10 metabolites were present in both the hepatopancreas and the muscle (five amino acids, 2-hydroxybutyrate, choline, myo-inositol, glycogen and uracil). 2-Hydroxyisobutyrate and creatine were present in both the hepatopancreas and the haemolymph. Phosphorylethanolamine, phosphocholine and fumarate were present only in the hepatopancreas and isoleucine only in the muscle. Statistical analysis showed that the percentage of weight gain was statistically higher in polyphenol-enriched diet groups compared to the control and that polyphenols had a stimulating effect on the general metabolism. No stress-related metabolites were higher in crayfish fed with polyphenol-enriched diet. Conversely, phosphatidylcholine, cholesterol and DHA, linked to resistance to environmental stress and diseases, were higher compared to the control diet. This study indicates that 1H-NMR is a useful tool to study the metabolomics in relation to diet differences.

LanguageEnglish
Pages524-538
Number of pages15
JournalAquaculture Nutrition
Volume24
Issue number1
DOIs
Publication statusPublished - Feb 1 2018

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Astacus leptodactylus
hepatopancreas
metabolomics
crayfish
nuclear magnetic resonance
polyphenols
muscle
diet
metabolite
hemolymph
muscles
metabolites
amino acid
cholesterol
uracil
environmental stress
creatine
phospholipid
amino acids
extracts

Keywords

  • H-NMR
  • crayfish
  • diets
  • haemolymph
  • hepatopancreas
  • metabolomics
  • muscle
  • polyphenols

ASJC Scopus subject areas

  • Aquatic Science

Cite this

1H-NMR metabolomic profiling of the crayfish Astacus leptodactylus subjected to polyphenol-enriched diets. / Costantini, Susan; Parrillo, Lucia; Guerriero, Eliana; Melck, Dominique; Colonna, Giovanni; Volpe, Maria Grazia; Paolucci, Marina.

In: Aquaculture Nutrition, Vol. 24, No. 1, 01.02.2018, p. 524-538.

Research output: Contribution to journalArticle

Costantini, Susan ; Parrillo, Lucia ; Guerriero, Eliana ; Melck, Dominique ; Colonna, Giovanni ; Volpe, Maria Grazia ; Paolucci, Marina. / 1H-NMR metabolomic profiling of the crayfish Astacus leptodactylus subjected to polyphenol-enriched diets. In: Aquaculture Nutrition. 2018 ; Vol. 24, No. 1. pp. 524-538.
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