1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola

Fabio Baroni, Luigia Pazzagli, Simone Luti, Aniello Scala, Federica Martellini, Lorella Franzoni, Thelma A. Pertinhez, Alberto Spisni

Research output: Contribution to journalArticle

Abstract

Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.

Original languageEnglish
Pages (from-to)405-408
Number of pages4
JournalBiomolecular NMR Assignments
Volume8
Issue number2
DOIs
Publication statusPublished - Oct 1 2014

Fingerprint

Plumbaginaceae
Proteins
Glucosamine
Cell death
Fungi
Cysteine
Genes
Chemical activation
Spectroscopy
Tissue
Pathogen-Associated Molecular Pattern Molecules
populin
Spectrum Analysis
Cell Death

Keywords

  • Defence inducers
  • Fungal elicitors
  • NMR
  • PAMP activity

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Medicine(all)

Cite this

Baroni, F., Pazzagli, L., Luti, S., Scala, A., Martellini, F., Franzoni, L., ... Spisni, A. (2014). 1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola. Biomolecular NMR Assignments, 8(2), 405-408. https://doi.org/10.1007/s12104-013-9527-x

1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola. / Baroni, Fabio; Pazzagli, Luigia; Luti, Simone; Scala, Aniello; Martellini, Federica; Franzoni, Lorella; Pertinhez, Thelma A.; Spisni, Alberto.

In: Biomolecular NMR Assignments, Vol. 8, No. 2, 01.10.2014, p. 405-408.

Research output: Contribution to journalArticle

Baroni, F, Pazzagli, L, Luti, S, Scala, A, Martellini, F, Franzoni, L, Pertinhez, TA & Spisni, A 2014, '1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola', Biomolecular NMR Assignments, vol. 8, no. 2, pp. 405-408. https://doi.org/10.1007/s12104-013-9527-x
Baroni, Fabio ; Pazzagli, Luigia ; Luti, Simone ; Scala, Aniello ; Martellini, Federica ; Franzoni, Lorella ; Pertinhez, Thelma A. ; Spisni, Alberto. / 1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola. In: Biomolecular NMR Assignments. 2014 ; Vol. 8, No. 2. pp. 405-408.
@article{68eb16f89cff475c8ce8a63597d4c03f,
title = "1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola",
abstract = "Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 {\%}. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.",
keywords = "Defence inducers, Fungal elicitors, NMR, PAMP activity",
author = "Fabio Baroni and Luigia Pazzagli and Simone Luti and Aniello Scala and Federica Martellini and Lorella Franzoni and Pertinhez, {Thelma A.} and Alberto Spisni",
year = "2014",
month = "10",
day = "1",
doi = "10.1007/s12104-013-9527-x",
language = "English",
volume = "8",
pages = "405--408",
journal = "Biomolecular NMR Assignments",
issn = "1874-2718",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - 1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola

AU - Baroni, Fabio

AU - Pazzagli, Luigia

AU - Luti, Simone

AU - Scala, Aniello

AU - Martellini, Federica

AU - Franzoni, Lorella

AU - Pertinhez, Thelma A.

AU - Spisni, Alberto

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.

AB - Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.

KW - Defence inducers

KW - Fungal elicitors

KW - NMR

KW - PAMP activity

UR - http://www.scopus.com/inward/record.url?scp=84940024211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940024211&partnerID=8YFLogxK

U2 - 10.1007/s12104-013-9527-x

DO - 10.1007/s12104-013-9527-x

M3 - Article

C2 - 24091893

AN - SCOPUS:84940024211

VL - 8

SP - 405

EP - 408

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

SN - 1874-2718

IS - 2

ER -