1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola

Fabio Baroni, Luigia Pazzagli, Simone Luti, Aniello Scala, Federica Martellini, Lorella Franzoni, Thelma A. Pertinhez, Alberto Spisni

Research output: Contribution to journalArticlepeer-review

Abstract

Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.

Original languageEnglish
Pages (from-to)405-408
Number of pages4
JournalBiomolecular NMR Assignments
Volume8
Issue number2
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Defence inducers
  • Fungal elicitors
  • NMR
  • PAMP activity

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Medicine(all)

Fingerprint Dive into the research topics of '<sup>1</sup>H, <sup>15</sup>N and <sup>13</sup>C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola'. Together they form a unique fingerprint.

Cite this