Abstract
Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
| Original language | English |
|---|---|
| Pages (from-to) | 405-408 |
| Number of pages | 4 |
| Journal | Biomolecular NMR Assignments |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 1 2014 |
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Keywords
- Defence inducers
- Fungal elicitors
- NMR
- PAMP activity
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Medicine(all)
Cite this
1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola. / Baroni, Fabio; Pazzagli, Luigia; Luti, Simone; Scala, Aniello; Martellini, Federica; Franzoni, Lorella; Pertinhez, Thelma A.; Spisni, Alberto.
In: Biomolecular NMR Assignments, Vol. 8, No. 2, 01.10.2014, p. 405-408.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola
AU - Baroni, Fabio
AU - Pazzagli, Luigia
AU - Luti, Simone
AU - Scala, Aniello
AU - Martellini, Federica
AU - Franzoni, Lorella
AU - Pertinhez, Thelma A.
AU - Spisni, Alberto
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
AB - Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20–C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-d-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
KW - Defence inducers
KW - Fungal elicitors
KW - NMR
KW - PAMP activity
UR - http://www.scopus.com/inward/record.url?scp=84940024211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84940024211&partnerID=8YFLogxK
U2 - 10.1007/s12104-013-9527-x
DO - 10.1007/s12104-013-9527-x
M3 - Article
C2 - 24091893
AN - SCOPUS:84940024211
VL - 8
SP - 405
EP - 408
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
SN - 1874-2718
IS - 2
ER -