99mTc-human serum albumin nanocolloids: Particle sizing and radioactivity distribution

Marco G. Persico, Lorenzo Lodola, Federica E. Buroni, Marco Morandotti, Piersandro Pallavicini, Carlo Aprile

Research output: Contribution to journalArticlepeer-review

Abstract

Several parameters affect the biodistribution of administered nanocolloids (NC) for Sentinel Lymph Node (SLN) detection: particle size distribution, number of Tc atoms per particle and specific activity (SA). Relatively few data are available with frequently conflicting results. 99mTc-NC-human serum albumin (HSA) Nanocoll®, Nanoalbumon® and Nanotop® were analysed for particles' dimensional and radioactivity distribution, and a mathematical model was elaborated to estimate the number of particles involved. Commercially available kits were reconstituted at maximal SA of 11MBq/μg HSA. Particles size distribution was evaluated by Dynamic Light Scattering. These data were related to the radioactivity distribution analysis passing labelled NC through three polycarbonate filters (15-30-50-nm pore size) under vacuum. Highest radioactivity was carried by 30-50nm particles. The smallest ones, even though most numerous, carried only the 10% of 99mTc atoms. Nanocoll and Nanotop are not significantly different, while Nanoalbumon is characterized by largest particles (>30nm) that carried the most of radioactivity (80%). Smallest particles could saturate the clearing capacity of macrophages; therefore, if the tracer is used for SLN detection, more node tiers could be visualized, reducing accuracy of SLN mapping. Manufacturers could implement technical leaflets with particle size distribution and could improve the labelling protocol to provide clinicians useful information. Three radiopharmaceuticals 99mTc-nanocolloids-human serum albumin (Nanocoll®, Nanoalbumon® and Nanotop®) were analysed for particles' dimensional and radioactivity distribution, and a mathematical model was elaborated to estimate the number of particles involved. These data were related to the radioactivity distribution analysis performed with nanofiltration. Highest radioactivity was carried by 30-50nm particles. The smallest ones, even though most numerous, carried only the 10% of 99mTc atoms. Nanocoll and Nanotop are not significantly different, while Nanoalbumon is characterized by largest particles that carried the most of radioactivity.

Original languageEnglish
Pages (from-to)376-382
Number of pages7
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume58
Issue number9
DOIs
Publication statusPublished - Jul 1 2015

Keywords

  • <sup>99m</sup>Tc radiopharmaceuticals
  • HSA nanocolloids
  • particle size
  • radioactivity distribution
  • Sentinel Lymph Node
  • SLN scintigraphy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Drug Discovery
  • Radiology Nuclear Medicine and imaging
  • Biochemistry

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