Superagonistic behaviour of epidermal growth factor/transforming growth factor-α chimaeras

Correlation with receptor routing after ligand-induced internalization

Anne E G Lenferink, Roelof H. Kramer, Marianne J H Van Vugt, Monika Königswieser, Pier Paolo Di Fiore, Everardus J J Van Zoelen, Monique L M Van De Poll

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Human epidermal growth factor (EGF) and human transforming growth factor alpha (TGF-α) are structurally related polypeptide growth factors that exert their mitogenic activity through interaction with a common cell-surface receptor, the epidermal growth factor receptor (EGFR). The biological effect induced by these two ligands is quantitatively similar in most cases; in some test systems, however, TGF-α functions as a more potent form of EGF. In this study, we have compared EGF, TGF-α and ten previously described chimaeras of these two ligands in terms of their ability to generate a mitogenic response in cells carrying the human EGFR, and observed that three of the mutant growth factors (E3T, E4T and T3E4T) are mitogenic at concentrations 10-fold lower than that of either wild-type EGF or TGF-α. No difference in tyrosine kinase activity of the receptor towards an external substrate was observed after binding of the various mutants. It has been established before that EGF and TGF-α differ in the processing of the receptor-ligand complex after internalization, as a result of their different pH sensitivities of receptor binding. Similar measurements on our chimaeric mutants revealed that the above superagonists show an enhanced pH dependence of binding in comparison with EGF. Furthermore, induction of receptor recycling by these superagonists is largely comparable with that induced by TGF-α. No superagonistic behaviour was observed on a cell-line containing an EGFR/erbB-2 chimaera which does not show ligand-induced internalization. These data show that EGF/TGFα chimaeras can be more active than the naturally occurring ligands, and that receptor recycling after ligand-induced internalization seems to be a prerequisite for this phenomenon.

Original languageEnglish
Pages (from-to)859-865
Number of pages7
JournalBiochemical Journal
Volume327
Issue number3
Publication statusPublished - Nov 1 1997

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Transforming Growth Factors
Epidermal Growth Factor
Transforming Growth Factor alpha
Ligands
Epidermal Growth Factor Receptor
Recycling
Intercellular Signaling Peptides and Proteins
Aptitude
Receptor Protein-Tyrosine Kinases
Cell Surface Receptors
Protein-Tyrosine Kinases
Cell Line
Peptides
Substrates
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lenferink, A. E. G., Kramer, R. H., Van Vugt, M. J. H., Königswieser, M., Di Fiore, P. P., Van Zoelen, E. J. J., & Van De Poll, M. L. M. (1997). Superagonistic behaviour of epidermal growth factor/transforming growth factor-α chimaeras: Correlation with receptor routing after ligand-induced internalization. Biochemical Journal, 327(3), 859-865.

Superagonistic behaviour of epidermal growth factor/transforming growth factor-α chimaeras : Correlation with receptor routing after ligand-induced internalization. / Lenferink, Anne E G; Kramer, Roelof H.; Van Vugt, Marianne J H; Königswieser, Monika; Di Fiore, Pier Paolo; Van Zoelen, Everardus J J; Van De Poll, Monique L M.

In: Biochemical Journal, Vol. 327, No. 3, 01.11.1997, p. 859-865.

Research output: Contribution to journalArticle

Lenferink, AEG, Kramer, RH, Van Vugt, MJH, Königswieser, M, Di Fiore, PP, Van Zoelen, EJJ & Van De Poll, MLM 1997, 'Superagonistic behaviour of epidermal growth factor/transforming growth factor-α chimaeras: Correlation with receptor routing after ligand-induced internalization', Biochemical Journal, vol. 327, no. 3, pp. 859-865.
Lenferink, Anne E G ; Kramer, Roelof H. ; Van Vugt, Marianne J H ; Königswieser, Monika ; Di Fiore, Pier Paolo ; Van Zoelen, Everardus J J ; Van De Poll, Monique L M. / Superagonistic behaviour of epidermal growth factor/transforming growth factor-α chimaeras : Correlation with receptor routing after ligand-induced internalization. In: Biochemical Journal. 1997 ; Vol. 327, No. 3. pp. 859-865.
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