Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor

M. Iacobini, G. Palumbo, S. Bartolozzi, C. Mondaini, M. Castello, A. Clerico, F. M. Perla, B. Werner, G. Digilio

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extracellular O2 - production in human polymorphonuclear cells (PMNs). Patients and Methods. Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 μg/kg/day). Intra- and extracellular O2 - production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and FMA stimulation. Results. All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2 - release after fMLP but not after PMA stimulation. Conclusions. rhG-CSF potentiates in vivo O2 - production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalHaematologica
Volume80
Issue number1
Publication statusPublished - 1995

Fingerprint

Granulocyte Colony-Stimulating Factor
Superoxides
Neutrophils
Neoplasms
Neutropenia
Precursor Cell Lymphoblastic Leukemia-Lymphoma
GTP-Binding Proteins
Drug Therapy
Therapeutics

Keywords

  • malignant tumors
  • PMNs
  • rhG-CSF
  • superoxide ion
  • tetrazolium salts

ASJC Scopus subject areas

  • Hematology

Cite this

Iacobini, M., Palumbo, G., Bartolozzi, S., Mondaini, C., Castello, M., Clerico, A., ... Digilio, G. (1995). Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor. Haematologica, 80(1), 13-17.

Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor. / Iacobini, M.; Palumbo, G.; Bartolozzi, S.; Mondaini, C.; Castello, M.; Clerico, A.; Perla, F. M.; Werner, B.; Digilio, G.

In: Haematologica, Vol. 80, No. 1, 1995, p. 13-17.

Research output: Contribution to journalArticle

Iacobini, M, Palumbo, G, Bartolozzi, S, Mondaini, C, Castello, M, Clerico, A, Perla, FM, Werner, B & Digilio, G 1995, 'Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor', Haematologica, vol. 80, no. 1, pp. 13-17.
Iacobini, M. ; Palumbo, G. ; Bartolozzi, S. ; Mondaini, C. ; Castello, M. ; Clerico, A. ; Perla, F. M. ; Werner, B. ; Digilio, G. / Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor. In: Haematologica. 1995 ; Vol. 80, No. 1. pp. 13-17.
@article{b8ac428820b5459aaa06d79c4fc5121e,
title = "Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor",
abstract = "Background. Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extracellular O2 - production in human polymorphonuclear cells (PMNs). Patients and Methods. Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 μg/kg/day). Intra- and extracellular O2 - production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and FMA stimulation. Results. All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2 - release after fMLP but not after PMA stimulation. Conclusions. rhG-CSF potentiates in vivo O2 - production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).",
keywords = "malignant tumors, PMNs, rhG-CSF, superoxide ion, tetrazolium salts",
author = "M. Iacobini and G. Palumbo and S. Bartolozzi and C. Mondaini and M. Castello and A. Clerico and Perla, {F. M.} and B. Werner and G. Digilio",
year = "1995",
language = "English",
volume = "80",
pages = "13--17",
journal = "Haematologica",
issn = "0390-6078",
publisher = "NLM (Medline)",
number = "1",

}

TY - JOUR

T1 - Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor

AU - Iacobini, M.

AU - Palumbo, G.

AU - Bartolozzi, S.

AU - Mondaini, C.

AU - Castello, M.

AU - Clerico, A.

AU - Perla, F. M.

AU - Werner, B.

AU - Digilio, G.

PY - 1995

Y1 - 1995

N2 - Background. Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extracellular O2 - production in human polymorphonuclear cells (PMNs). Patients and Methods. Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 μg/kg/day). Intra- and extracellular O2 - production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and FMA stimulation. Results. All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2 - release after fMLP but not after PMA stimulation. Conclusions. rhG-CSF potentiates in vivo O2 - production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).

AB - Background. Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extracellular O2 - production in human polymorphonuclear cells (PMNs). Patients and Methods. Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 μg/kg/day). Intra- and extracellular O2 - production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and FMA stimulation. Results. All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2 - release after fMLP but not after PMA stimulation. Conclusions. rhG-CSF potentiates in vivo O2 - production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).

KW - malignant tumors

KW - PMNs

KW - rhG-CSF

KW - superoxide ion

KW - tetrazolium salts

UR - http://www.scopus.com/inward/record.url?scp=0028928232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028928232&partnerID=8YFLogxK

M3 - Article

C2 - 7538966

AN - SCOPUS:0028928232

VL - 80

SP - 13

EP - 17

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 1

ER -