Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor

M. Iacobini, G. Palumbo, S. Bartolozzi, C. Mondaini, M. Castello, A. Clerico, F. M. Perla, B. Werner, G. Digilio

Research output: Contribution to journalArticle

Abstract

Background. Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extracellular O2 - production in human polymorphonuclear cells (PMNs). Patients and Methods. Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 μg/kg/day). Intra- and extracellular O2 - production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and FMA stimulation. Results. All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2 - release after fMLP but not after PMA stimulation. Conclusions. rhG-CSF potentiates in vivo O2 - production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalHaematologica
Volume80
Issue number1
Publication statusPublished - 1995

Keywords

  • malignant tumors
  • PMNs
  • rhG-CSF
  • superoxide ion
  • tetrazolium salts

ASJC Scopus subject areas

  • Hematology

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    Iacobini, M., Palumbo, G., Bartolozzi, S., Mondaini, C., Castello, M., Clerico, A., Perla, F. M., Werner, B., & Digilio, G. (1995). Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor. Haematologica, 80(1), 13-17.