Suppression of 3-hydroxy-3-methylglutaryl-CoA reductase by low density lipoproteins produced in vitro by lipoprotein lipase action on nonsuppressive very low density lipoproteins

A. L. Catapano, S. H. Gianturco, P. K J Kinnunen, S. Eisenberg, A. M. Gotto, L. C. Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Very low density lipoproteins (VLDL), S(f)60 to 400, from normolipemic individuals do not suppress 3-hydroxy-3-methylglutaryl-CoA reductase activity in cultured normal human fibroblasts at concentrations 20-fold higher than those of low density lipoproteins (LDL) that give total suppression. To determine if these VLDL contain all of the structural elements necessary for receptor-mediated suppression, they were converted in vitro with bovine milk lipoprotein lipase to low density lipoproteins. These LDL-like lipoproteins were as effective in suppression as LDL isolated directly from plasma, with half-maximal and complete suppression at 1 and 4 μg of cholesterol ml-1. Neither native LDL nor LDL produced in vitro suppressed receptor-negative fibroblasts. It is concluded that action of lipoprotein lipase on VLDL leads to a rearrangement of lipoprotein components that permits interaction of LDL produced in vitro with the LDL-specific cell surface receptor of fibroblasts and subsequent suppression of 3-hydroxy-3-methylglutaryl-CoA reductase.

Original languageEnglish
Pages (from-to)1007-1009
Number of pages3
JournalJournal of Biological Chemistry
Volume254
Issue number4
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Biochemistry

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