Suppression of natural killer cell function and production of soluble ICAM-1

Endometrial stroma versus melanoma

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Problem: It has been suggested that specific mechanisms commonly used by different cellular systems to evade immunologic recognition are involved in the development of endometriosis. To gain insight into this aspect, we looked at the relationship between two of these mechanisms in endometrial stroma and the melanoma system for which the ability to create an environment of immune privilege has been well established. Method of study: Media conditioned by endometrial stromal cultures and malignant melanoma A375 were examined to test their effects on peripheral blood mononuclear cell-mediated cytotoxicity directed against K562 target. Moreover, these media were tested for the concentration of the soluble form of intercellular adhesion molecule-1 (sICAM-1), which has been suggested as a marker for spreading potential. Results: Media conditioned by endometrial stromal cultures exerted a significant suppressive effect on cell cytotoxicity when compared with those derived from malignant melanoma. Moreover, the constitutive release of sICAM-1 was significantly higher in supernatants from endometrial stromal than in melanoma cells. Conclusions: These results indicate that two specific properties suggested to be involved in the ability of tumor cells to evade the immune system are more pronounced in the endometrium than in a malignant melanoma. Since the properties evaluated have been previously demonstrated to be even more notable in endometrial samples derived from endometriosis patients, a role of these mechanisms in the development of the disease may be hypothesized.

Original languageEnglish
Pages (from-to)342-348
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume46
Issue number5
Publication statusPublished - 2001

Fingerprint

Intercellular Adhesion Molecule-1
Natural Killer Cells
Melanoma
Endometriosis
Conditioned Culture Medium
Endometrium
Immune System
Blood Cells
Neoplasms

Keywords

  • Endometrium
  • Immune system
  • Melanoma
  • NK cells
  • Soluble intercellular adhesion molecule-1

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

Cite this

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title = "Suppression of natural killer cell function and production of soluble ICAM-1: Endometrial stroma versus melanoma",
abstract = "Problem: It has been suggested that specific mechanisms commonly used by different cellular systems to evade immunologic recognition are involved in the development of endometriosis. To gain insight into this aspect, we looked at the relationship between two of these mechanisms in endometrial stroma and the melanoma system for which the ability to create an environment of immune privilege has been well established. Method of study: Media conditioned by endometrial stromal cultures and malignant melanoma A375 were examined to test their effects on peripheral blood mononuclear cell-mediated cytotoxicity directed against K562 target. Moreover, these media were tested for the concentration of the soluble form of intercellular adhesion molecule-1 (sICAM-1), which has been suggested as a marker for spreading potential. Results: Media conditioned by endometrial stromal cultures exerted a significant suppressive effect on cell cytotoxicity when compared with those derived from malignant melanoma. Moreover, the constitutive release of sICAM-1 was significantly higher in supernatants from endometrial stromal than in melanoma cells. Conclusions: These results indicate that two specific properties suggested to be involved in the ability of tumor cells to evade the immune system are more pronounced in the endometrium than in a malignant melanoma. Since the properties evaluated have been previously demonstrated to be even more notable in endometrial samples derived from endometriosis patients, a role of these mechanisms in the development of the disease may be hypothesized.",
keywords = "Endometrium, Immune system, Melanoma, NK cells, Soluble intercellular adhesion molecule-1",
author = "Paola Vigan{\`o} and Edgardo Somigliana and {Di Blasio}, {Anna Maria} and Sabrina Cozzolino and Massimo Candiani and Mario Vignali",
year = "2001",
language = "English",
volume = "46",
pages = "342--348",
journal = "American Journal of Reproductive Immunology and Microbiology",
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TY - JOUR

T1 - Suppression of natural killer cell function and production of soluble ICAM-1

T2 - Endometrial stroma versus melanoma

AU - Viganò, Paola

AU - Somigliana, Edgardo

AU - Di Blasio, Anna Maria

AU - Cozzolino, Sabrina

AU - Candiani, Massimo

AU - Vignali, Mario

PY - 2001

Y1 - 2001

N2 - Problem: It has been suggested that specific mechanisms commonly used by different cellular systems to evade immunologic recognition are involved in the development of endometriosis. To gain insight into this aspect, we looked at the relationship between two of these mechanisms in endometrial stroma and the melanoma system for which the ability to create an environment of immune privilege has been well established. Method of study: Media conditioned by endometrial stromal cultures and malignant melanoma A375 were examined to test their effects on peripheral blood mononuclear cell-mediated cytotoxicity directed against K562 target. Moreover, these media were tested for the concentration of the soluble form of intercellular adhesion molecule-1 (sICAM-1), which has been suggested as a marker for spreading potential. Results: Media conditioned by endometrial stromal cultures exerted a significant suppressive effect on cell cytotoxicity when compared with those derived from malignant melanoma. Moreover, the constitutive release of sICAM-1 was significantly higher in supernatants from endometrial stromal than in melanoma cells. Conclusions: These results indicate that two specific properties suggested to be involved in the ability of tumor cells to evade the immune system are more pronounced in the endometrium than in a malignant melanoma. Since the properties evaluated have been previously demonstrated to be even more notable in endometrial samples derived from endometriosis patients, a role of these mechanisms in the development of the disease may be hypothesized.

AB - Problem: It has been suggested that specific mechanisms commonly used by different cellular systems to evade immunologic recognition are involved in the development of endometriosis. To gain insight into this aspect, we looked at the relationship between two of these mechanisms in endometrial stroma and the melanoma system for which the ability to create an environment of immune privilege has been well established. Method of study: Media conditioned by endometrial stromal cultures and malignant melanoma A375 were examined to test their effects on peripheral blood mononuclear cell-mediated cytotoxicity directed against K562 target. Moreover, these media were tested for the concentration of the soluble form of intercellular adhesion molecule-1 (sICAM-1), which has been suggested as a marker for spreading potential. Results: Media conditioned by endometrial stromal cultures exerted a significant suppressive effect on cell cytotoxicity when compared with those derived from malignant melanoma. Moreover, the constitutive release of sICAM-1 was significantly higher in supernatants from endometrial stromal than in melanoma cells. Conclusions: These results indicate that two specific properties suggested to be involved in the ability of tumor cells to evade the immune system are more pronounced in the endometrium than in a malignant melanoma. Since the properties evaluated have been previously demonstrated to be even more notable in endometrial samples derived from endometriosis patients, a role of these mechanisms in the development of the disease may be hypothesized.

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KW - Immune system

KW - Melanoma

KW - NK cells

KW - Soluble intercellular adhesion molecule-1

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