Surface distribution and internalization of erbB-2 Proteins

Lavinia Vittoria Lotti, Claudia Di Lazzaro, Claudia Zompetta, Luigi Frati, Maria Rosaria Torrisi

Research output: Contribution to journalArticle

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Abstract

We report the localization over the cell surface and the early steps of antibody-induced internalization of the product of the erbB-2 proto-oncogene, structurally related to the epidermal growth factor receptor (EGFR). We show that erbB-2/p185 is mostly excluded from endocytic pits on the cell surface. Incubation at 37 °C with an anti-erbB-2/p185 monoclonal antibody induces the rapid entry of the protein into the cell. Similar internalization is shown by a chimeric molecule EGFB/erbB-2 in response to EGF. Both the timing and the pathway of internalization followed by the erbB-2/ p185 appear totally similar to those described for the EGFR. At variance with the normal erbB-2/p185, two mutant activated erbB-2 proteins are frequently localized within endocytic pits of the cell surface, indicating that mutations in the transmembrane regions may determine constitutive internalization of the protein.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalExperimental Cell Research
Volume202
Issue number2
DOIs
Publication statusPublished - 1992

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Epidermal Growth Factor Receptor
Proteins
Proto-Oncogenes
Epidermal Growth Factor
Monoclonal Antibodies
Mutation
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Surface distribution and internalization of erbB-2 Proteins. / Lotti, Lavinia Vittoria; Di Lazzaro, Claudia; Zompetta, Claudia; Frati, Luigi; Torrisi, Maria Rosaria.

In: Experimental Cell Research, Vol. 202, No. 2, 1992, p. 274-280.

Research output: Contribution to journalArticle

Lotti, LV, Di Lazzaro, C, Zompetta, C, Frati, L & Torrisi, MR 1992, 'Surface distribution and internalization of erbB-2 Proteins', Experimental Cell Research, vol. 202, no. 2, pp. 274-280. https://doi.org/10.1016/0014-4827(92)90075-J
Lotti, Lavinia Vittoria ; Di Lazzaro, Claudia ; Zompetta, Claudia ; Frati, Luigi ; Torrisi, Maria Rosaria. / Surface distribution and internalization of erbB-2 Proteins. In: Experimental Cell Research. 1992 ; Vol. 202, No. 2. pp. 274-280.
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