Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes

Laura Rigamonti, Silvia Ariotti, Giuliana Losana, Roberto Gradini, Matteo A. Russo, Emmanuelle Jouanguy, Jean Laurent Casanova, Guido Forni, Francesco Novelli

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Abstract

The surface and cytoplasmic expressions of the transducing chain (IFN- γR2) of the heterodimeric IFN-γ receptor on human T lymphocytes have been investigated. We show that its surface expression is low, whereas high cytoplasmic levels are found in both resting and PHA-activated T lymphocytes. This low expression does not prevent activated T cells from responding to IFN-γ, because it induces IFN-regulatory factor 1 expression. Low surface IFN-γR2 expression appears to be due to recycling between cytoplasmic stores and the cell surface, which does not depend on signals mediated by endogenous IFN-γ, because IFN-γR2 surface expression is low, and its internalization is equally observed in patients with inherited IFN-γR1 gene deficiency and in healthy donors. Moreover, IFN-γR2 internalization in T lymphoblasts from healthy donors was not affected by the presence of anti-IFN-γ-neutralizing or anti-IFN-γR1-blocking mAb. In conclusion, these data illustrate a new mechanism whereby human T cells limit the surface expression of IFN-γR2 in a ligand-independent manner.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Immunology
Volume164
Issue number1
Publication statusPublished - Jan 1 2000

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Human Trafficking
T-Lymphocytes
Tissue Donors
Recycling
Ligands
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Rigamonti, L., Ariotti, S., Losana, G., Gradini, R., Russo, M. A., Jouanguy, E., ... Novelli, F. (2000). Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes. Journal of Immunology, 164(1), 201-207.

Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes. / Rigamonti, Laura; Ariotti, Silvia; Losana, Giuliana; Gradini, Roberto; Russo, Matteo A.; Jouanguy, Emmanuelle; Casanova, Jean Laurent; Forni, Guido; Novelli, Francesco.

In: Journal of Immunology, Vol. 164, No. 1, 01.01.2000, p. 201-207.

Research output: Contribution to journalArticle

Rigamonti, L, Ariotti, S, Losana, G, Gradini, R, Russo, MA, Jouanguy, E, Casanova, JL, Forni, G & Novelli, F 2000, 'Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes', Journal of Immunology, vol. 164, no. 1, pp. 201-207.
Rigamonti L, Ariotti S, Losana G, Gradini R, Russo MA, Jouanguy E et al. Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes. Journal of Immunology. 2000 Jan 1;164(1):201-207.
Rigamonti, Laura ; Ariotti, Silvia ; Losana, Giuliana ; Gradini, Roberto ; Russo, Matteo A. ; Jouanguy, Emmanuelle ; Casanova, Jean Laurent ; Forni, Guido ; Novelli, Francesco. / Surface expression of the IFN-ΓR2 chain is regulated by intracellular trafficking in human T lymphocytes. In: Journal of Immunology. 2000 ; Vol. 164, No. 1. pp. 201-207.
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