Surface film formation in vitro by infant and therapeutic surfactants

Role of surfactant protein B

Olivier Danhaive, Cheryl Chapin, Hart Horneman, Paola E. Cogo, Philip L. Ballard

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background:Pulmonary surfactant provides an alveolar surface-active film that is critical for normal lung function. Our objective was to determine in vitro film formation properties of therapeutic and infant surfactants and the influence of surfactant protein (SP)-B content.Methods:We used a multiwell fluorescent assay measuring maximum phospholipid surface accumulation (Max), phospholipid concentration required for half-maximal film formation (Max), and time for maximal accumulation (tMax).Results:Among five therapeutic surfactants, calfactant (highest SP-B content) had film formation values similar to natural surfactant, and addition of SP-B to beractant (lowest SP-B) normalized its Max value. Addition of budesonide to calfactant did not adversely affect film formation. In tracheal aspirates of preterm infants with evolving chronic lung disease, SP-B content correlated with Max and tMax values, and SP-B supplementation of SP-B-deficient infant surfactant restored normal film formation. Reconstitution of normal surfactant indicated a role for both SP-B and SP-C in film formation.Conclusion:Film formation in vitro differs among therapeutic surfactants and is highly dependent on SP-B content in infant surfactant. The results support a critical role of SP-B for promoting surface film formation.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalPediatric Research
Volume77
Issue number2
DOIs
Publication statusPublished - Feb 11 2015

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Surface-Active Agents
Therapeutics
In Vitro Techniques
IgA receptor
Phospholipids
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants
Budesonide
Protein C
Premature Infants
Lung Diseases

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Surface film formation in vitro by infant and therapeutic surfactants : Role of surfactant protein B. / Danhaive, Olivier; Chapin, Cheryl; Horneman, Hart; Cogo, Paola E.; Ballard, Philip L.

In: Pediatric Research, Vol. 77, No. 2, 11.02.2015, p. 340-346.

Research output: Contribution to journalArticle

Danhaive, Olivier ; Chapin, Cheryl ; Horneman, Hart ; Cogo, Paola E. ; Ballard, Philip L. / Surface film formation in vitro by infant and therapeutic surfactants : Role of surfactant protein B. In: Pediatric Research. 2015 ; Vol. 77, No. 2. pp. 340-346.
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