Immunoglobuline di superficie (SmIg) nei linfociti B nell'uomo: effetto della digestione enzimatica.

E. Mariani; G. F. Stefanini; A. Ferruzzi; M. Garagnani; A. Facchini

Immunoglobuline di superficie (SmIg) nei linfociti B nell'uomo: effetto della digestione enzimatica.

Translated title of the contribution: Surface immunoglobulins (SmIg) in human B-lymphocytes: effect of enzymatic digestion

E. Mariani, G. F. Stefanini, A. Ferruzzi, M. Garagnani, A. Facchini

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

In order to determine the number of circulating B lymphocytes which can synthetize their surface membrane immunoglobulins, we have evaluated by immunofluorescence their presence before and after enzymatic exposure to pronase. After pronase treatment the majority of lymphocytes carrying SmIg has been disappeared (from 7.8% to 1.9%; p <.0.0005). After 18 hours incubaton only a small proportion (4.6%) of B lymphocytes risynthetized their SmIg. This observation support the hypothesis that a subset of lymphocytes present in the peripheral blood carries on its membrane only cytophilic SmIg and therefore may interfere in the determination of the number of B lymphocytes.

Translated title of the contribution	Surface immunoglobulins (SmIg) in human B-lymphocytes: effect of enzymatic digestion
Original language	Italian
Pages (from-to)	411-416
Number of pages	6
Journal	Bollettino della Societa Italiana di Biologia Sperimentale
Volume	55
Issue number	5
Publication status	Published - Mar 15 1979

ASJC Scopus subject areas

Medicine(all)

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title = "Immunoglobuline di superficie (SmIg) nei linfociti B nell'uomo: effetto della digestione enzimatica.",

abstract = "In order to determine the number of circulating B lymphocytes which can synthetize their surface membrane immunoglobulins, we have evaluated by immunofluorescence their presence before and after enzymatic exposure to pronase. After pronase treatment the majority of lymphocytes carrying SmIg has been disappeared (from 7.8% to 1.9%; p <.0.0005). After 18 hours incubaton only a small proportion (4.6%) of B lymphocytes risynthetized their SmIg. This observation support the hypothesis that a subset of lymphocytes present in the peripheral blood carries on its membrane only cytophilic SmIg and therefore may interfere in the determination of the number of B lymphocytes.",

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AU - Stefanini, G. F.

AU - Ferruzzi, A.

AU - Garagnani, M.

AU - Facchini, A.

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Immunoglobuline di superficie (SmIg) nei linfociti B nell'uomo: effetto della digestione enzimatica.

Abstract

ASJC Scopus subject areas

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