Abstract
Two approaches were used to prepare a series of surface-modified capillaries. In the first, a sublayer was formed by coupling γ-methacryloxypropyltrimethoxysilane to the surface silanol groups forming an SI-O bond; a top layer was then formed by polymerizing acrylamide in the capillary, which reacted with the sublayer. In the second approach, a sublayer was formed by silanol chlorination, followed by Grignard coupling of vinylmagnesium bromide to form an Si-C bond at the surface; a top layer was formed by polymerizing either. acrylamide (AA), dimethylacrylamide (DMA), N-acryloylaminoethoxyethanol (AAEE), or N-acryloylaminopropanol (AAP) onto the sublayer. The Si-C-poly(AA) capillaries were more stable and produced an approximately 10-fold lower electroosmotic flow compared to the Si-O-poly(AA) capillaries. The Si-C sublayer was used to compare the performance of all four top layers. Electroosmotic flow decreased in the order: Si-O-poly(AA), Si-C-poly(AA), Si-C-poly(AAEE), Si-C-poly(DMA), and Si-C-poly(AAP). Si-C-poly(AA) showed evidence of irreversible degradation at pH 9 already after 40-50 runs. Si-C-polyAAP-coated capillaries demonstrated superior efficiency and migration time reproducibility for a number of alkaline proteins and for fluorescently labeled ovalbumin. Excellent performance was maintained, in the case of poly(AAP), for a least 300 runs (of 30 min duration) at pH 9.0.
| Original language | English |
|---|---|
| Pages (from-to) | 1677-1682 |
| Number of pages | 6 |
| Journal | Electrophoresis |
| Volume | 19 |
| Issue number | 10 |
| Publication status | Published - Jul 1998 |
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Keywords
- Capillary electrophoresis
- Coating
- Ekectroosmosis
- N-substituted acrylamide
- Silica
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Surface modification based on Si-O and Si-C sublayers and a series of N-substituted acrylamide top-layers for capillary electrophoresis. / Gelfi, Cecilia; Curcio, Mario; Righetti, Pier Giorgio; Sebastiano, Roberto; Citterio, Attilio; Ahmadzadeh, Hossein; Dovichi, Norman J.
In: Electrophoresis, Vol. 19, No. 10, 07.1998, p. 1677-1682.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface modification based on Si-O and Si-C sublayers and a series of N-substituted acrylamide top-layers for capillary electrophoresis
AU - Gelfi, Cecilia
AU - Curcio, Mario
AU - Righetti, Pier Giorgio
AU - Sebastiano, Roberto
AU - Citterio, Attilio
AU - Ahmadzadeh, Hossein
AU - Dovichi, Norman J.
PY - 1998/7
Y1 - 1998/7
N2 - Two approaches were used to prepare a series of surface-modified capillaries. In the first, a sublayer was formed by coupling γ-methacryloxypropyltrimethoxysilane to the surface silanol groups forming an SI-O bond; a top layer was then formed by polymerizing acrylamide in the capillary, which reacted with the sublayer. In the second approach, a sublayer was formed by silanol chlorination, followed by Grignard coupling of vinylmagnesium bromide to form an Si-C bond at the surface; a top layer was formed by polymerizing either. acrylamide (AA), dimethylacrylamide (DMA), N-acryloylaminoethoxyethanol (AAEE), or N-acryloylaminopropanol (AAP) onto the sublayer. The Si-C-poly(AA) capillaries were more stable and produced an approximately 10-fold lower electroosmotic flow compared to the Si-O-poly(AA) capillaries. The Si-C sublayer was used to compare the performance of all four top layers. Electroosmotic flow decreased in the order: Si-O-poly(AA), Si-C-poly(AA), Si-C-poly(AAEE), Si-C-poly(DMA), and Si-C-poly(AAP). Si-C-poly(AA) showed evidence of irreversible degradation at pH 9 already after 40-50 runs. Si-C-polyAAP-coated capillaries demonstrated superior efficiency and migration time reproducibility for a number of alkaline proteins and for fluorescently labeled ovalbumin. Excellent performance was maintained, in the case of poly(AAP), for a least 300 runs (of 30 min duration) at pH 9.0.
AB - Two approaches were used to prepare a series of surface-modified capillaries. In the first, a sublayer was formed by coupling γ-methacryloxypropyltrimethoxysilane to the surface silanol groups forming an SI-O bond; a top layer was then formed by polymerizing acrylamide in the capillary, which reacted with the sublayer. In the second approach, a sublayer was formed by silanol chlorination, followed by Grignard coupling of vinylmagnesium bromide to form an Si-C bond at the surface; a top layer was formed by polymerizing either. acrylamide (AA), dimethylacrylamide (DMA), N-acryloylaminoethoxyethanol (AAEE), or N-acryloylaminopropanol (AAP) onto the sublayer. The Si-C-poly(AA) capillaries were more stable and produced an approximately 10-fold lower electroosmotic flow compared to the Si-O-poly(AA) capillaries. The Si-C sublayer was used to compare the performance of all four top layers. Electroosmotic flow decreased in the order: Si-O-poly(AA), Si-C-poly(AA), Si-C-poly(AAEE), Si-C-poly(DMA), and Si-C-poly(AAP). Si-C-poly(AA) showed evidence of irreversible degradation at pH 9 already after 40-50 runs. Si-C-polyAAP-coated capillaries demonstrated superior efficiency and migration time reproducibility for a number of alkaline proteins and for fluorescently labeled ovalbumin. Excellent performance was maintained, in the case of poly(AAP), for a least 300 runs (of 30 min duration) at pH 9.0.
KW - Capillary electrophoresis
KW - Coating
KW - Ekectroosmosis
KW - N-substituted acrylamide
KW - Silica
UR - http://www.scopus.com/inward/record.url?scp=0031858139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031858139&partnerID=8YFLogxK
M3 - Article
C2 - 9719545
AN - SCOPUS:0031858139
VL - 19
SP - 1677
EP - 1682
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 10
ER -